Yang Ting, Qiu Lingling, Chen Shihao, Wang Zhixiu, Jiang Yong, Bai Hao, Bi Yulin, Chang Guobin
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, Yangzhou University, Yangzhou 225009, China.
Animals (Basel). 2024 Aug 28;14(17):2500. doi: 10.3390/ani14172500.
Avian Leukosis virus (ALV) is a widely spread virus that causes major economic losses to the global poultry industry. This study aims to investigate the effect of glycolysis on the replication of the ALV-J virus and identify the key circular RNAs that regulate the replication of the ALV-J virus. We found that glucose uptake, pyruvate content, and lactate content in DF1 cells were increased after ALV-J infection. Moreover, inhibiting the glycolysis of ALV-J-infected DF1 cells reduced the replication of the ALV-J virus. To further study the mechanism of glycolysis in the replication of the ALV-J virus, we performed RNA-seq on ALV-J-infected and ALV-J-infected cells treated with glycolysis inhibition. RNA-seq results show that a total of 10,375 circular RNAs (circRNAs) were identified, of which the main types were exonic circular RNAs, and 28 circRNAs were differentially expressed between ALV-J-infected and ALV-J-infected cells treated with glycolysis inhibition. Then, we performed functional enrichment analysis of differentially expressed circRNA source and target genes. Functional enrichment analysis indicated that some circRNAs might be involved in regulating the replication of the ALV-J virus by influencing some pathways like glycolysis/gluconeogenesis, the NOD-like receptor signaling pathway, MAPK signaling pathway, p53 signaling pathway, Toll-like receptor signaling pathway, Insulin signaling pathway, and Apoptosis. This study revealed the effect of glycolysis on the replication of the ALV-J virus in DF1 cells and its possible regulatory mechanism, which provided a basis for understanding the factors influencing the replication of the ALV-J virus and reducing the rate of infection of the ALV-J virus in poultry.
禽白血病病毒（ALV）是一种广泛传播的病毒，给全球家禽业造成重大经济损失。本研究旨在探讨糖酵解对ALV-J病毒复制的影响，并鉴定调控ALV-J病毒复制的关键环状RNA。我们发现，DF1细胞在感染ALV-J后，葡萄糖摄取量、丙酮酸含量和乳酸含量均增加。此外，抑制ALV-J感染的DF1细胞的糖酵解可降低ALV-J病毒的复制。为进一步研究糖酵解在ALV-J病毒复制中的机制，我们对ALV-J感染的细胞以及用糖酵解抑制剂处理的ALV-J感染细胞进行了RNA测序。RNA测序结果显示，共鉴定出10375个环状RNA（circRNA），其中主要类型为外显子环状RNA，在ALV-J感染的细胞和用糖酵解抑制剂处理的ALV-J感染细胞之间有28个circRNA差异表达。然后，我们对差异表达的circRNA来源和靶基因进行了功能富集分析。功能富集分析表明，一些circRNA可能通过影响糖酵解/糖异生、NOD样受体信号通路、MAPK信号通路、p53信号通路、Toll样受体信号通路、胰岛素信号通路和凋亡等途径参与调控ALV-J病毒的复制。本研究揭示了糖酵解对DF1细胞中ALV-J病毒复制的影响及其可能的调控机制，为了解影响ALV-J病毒复制的因素和降低家禽中ALV-J病毒的感染率提供了依据。
