Alvarez-González C A, Moyano-López F J, Civera-Cerecedo R, Carrasco-Chávez V, Ortiz-Galindo J L, Dumas S
DACBIOL Laboratorio de Acuacultura, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, Mexico.
Fish Physiol Biochem. 2008 Dec;34(4):373-84. doi: 10.1007/s10695-007-9197-7. Epub 2008 Feb 7.
Spotted sand bass Paralabrax maculatofasciatus is a potential aquaculture species in Northwest Mexico. In the last few years it has been possible to close its life cycle and to develop larviculture technology at on pilot scale using live food, however survival values are low (11%) and improvements in growth and survival requires the study of the morpho-physiological development during the initial ontogeny. In this research digestive activity of several enzymes were evaluated in larvae, from hatching to 30 days after hatching (dah), and in live prey (rotifers and Artemia), by use of biochemical and electrophoretic techniques. This paper, is the first of two parts, and covers only the biochemical analysis. All digestive enzyme activities were detected from mouth opening; however the, maximum activities varied among different digestive enzymes. For alkaline protease and trypsin the maximum activities were detected from 12 to 18 dah. Acid protease activity was observed from day 12 onwards. The other digestive enzymes appear between days 4 and 18 after hatching, with marked fluctuations. These activities indicate the beginning of the juvenile stage and the maturation of the digestive system, in agreement with changes that occur during morpho-physiological development and food changes from rotifers to Artemia. All enzymatic activities were detected in rotifers and Artemia, and their contribution to enhancement the digestion capacity of the larvae appears to be low, but cannot be minimised. We concluded that the enzymatic equipment of P. maculatofasciatus larvae is similar to that of other marine fish species, that it becomes complete between days 12 and 18 after hatching, and that it is totally efficient up to 25 dah.
斑点砂鲈（Paralabrax maculatofasciatus）是墨西哥西北部一种具有养殖潜力的鱼类。在过去几年里，已经能够完成其生命周期，并在中试规模上利用活饵开发幼鱼养殖技术，然而存活率较低（11%），要提高生长率和存活率，需要研究其早期个体发育过程中的形态生理发育情况。在本研究中，利用生化和电泳技术，对斑点砂鲈幼鱼从孵化到孵化后30天（dah）以及活饵（轮虫和卤虫）中的几种酶的消化活性进行了评估。本文是两部分中的第一部分，仅涵盖生化分析。所有消化酶活性在开口时即可检测到；然而，不同消化酶的最大活性有所不同。碱性蛋白酶和胰蛋白酶的最大活性在12至18 dah时检测到。酸性蛋白酶活性从第12天开始观察到。其他消化酶在孵化后4至18天之间出现，且有明显波动。这些活性表明幼鱼阶段的开始和消化系统的成熟，这与形态生理发育过程中以及食物从轮虫转变为卤虫时发生的变化相一致。在轮虫和卤虫中均检测到所有酶活性，它们对提高幼鱼消化能力的贡献似乎较低，但不能忽视。我们得出结论，斑点砂鲈幼鱼的酶装备与其他海水鱼类相似，在孵化后12至18天之间变得完整，并且在25 dah之前完全有效。
