Kaneko T, Nakano S, Matsuo M
Tokyo Metropolitan Institute of Gerontology, Japan.
Lipids. 1991 May;26(5):345-8. doi: 10.1007/BF02537196.
The protective effects of vitamin E (alpha-tocopherol) and its model compounds, which act as antioxidants, on linoleic acid hydroperoxide-induced injury to human umbilical vein endothelial cells were examined. When incubated at 50 microM with endothelial cells at 37 degrees C for 24 hr, alpha-tocopherol protected the cells from injury, and 2,2,5,7,8-pentamethylchroman-6-ol showed a similar protective effect. Trolox C, a water-soluble vitamin E model compound, had no protective effect. Tocol, a poor antioxidant, proved toxic. During preincubation, alpha-tocopherol was incorporated into the cells at 16.6 nmol/mg protein, while the pentamethylchromanol was incorporated at 0.5 nmol/mg protein; Trolox C was not incorporated at all. The results suggest that agents having both high antioxidant activity and lipophilicity can protect endothelial cells from linoleic acid hydroperoxide-induced injury.
研究了作为抗氧化剂的维生素E（α-生育酚）及其模型化合物对亚油酸氢过氧化物诱导的人脐静脉内皮细胞损伤的保护作用。当在37℃下与内皮细胞以50μM孵育24小时时，α-生育酚保护细胞免受损伤，并且2,2,5,7,8-五甲基色满-6-醇显示出类似的保护作用。水溶性维生素E模型化合物Trolox C没有保护作用。生育酚，一种较差的抗氧化剂，被证明具有毒性。在预孵育期间，α-生育酚以16.6 nmol/mg蛋白质的量掺入细胞中，而五甲基色满醇以0.5 nmol/mg蛋白质的量掺入；Trolox C根本没有掺入。结果表明，具有高抗氧化活性和亲脂性的试剂可以保护内皮细胞免受亚油酸氢过氧化物诱导的损伤。
