采用液-液萃取结合毛细管电泳同时进行电化学和电化学发光检测法分析尿液中的苯丙胺类药物。
Analysis of amphetamines in urine with liquid-liquid extraction by capillary electrophoresis with simultaneous electrochemical and electrochemiluminescence detection.
作者信息
Sun Jinying, Xu Xiaoyu, Wang Chunyan, You Tianyan
机构信息
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Changchun, P. R. China.
出版信息
Electrophoresis. 2008 Oct;29(19):3999-4007. doi: 10.1002/elps.200700875.
Amphetamines including methamphetamine, 3,4-methylenedioxyamphetamine and 3,4-methylenedioxymethamphetamine were separated and detected by CE using simultaneous electrochemical (EC) and electrochemiluminescence (ECL) detection (CE-EC/ECL). Factors that influenced the separation and detection performance, such as the detection potential, the pH value and concentration of the running buffer, the separation voltage and the pH of the detection buffer, were investigated. LODs of 3.3x10(-8) mol/L (0.16 fmol), 1.6x10(-7) mol/L (0.78 fmol) and 3.3x10(-8) mol/L (0.16 fmol) were obtained for methamphetamine, 3,4-methylenedioxyamphetamine and 3,4-methylenedioxymethamphetamine, respectively. For practical application, a liquid-liquid extraction with ethyl acetate procedure was developed for urine sample pretreatment and extraction efficiencies higher than 90% were obtained. The established simultaneous CE-EC/ECL was successfully applied for urine sample analysis.
包括甲基苯丙胺、3,4-亚甲基二氧基苯丙胺和3,4-亚甲基二氧基甲基苯丙胺在内的苯丙胺类药物，通过毛细管电泳（CE）结合同步电化学（EC）和电化学发光（ECL）检测（CE-EC/ECL）进行分离和检测。研究了影响分离和检测性能的因素，如检测电位、运行缓冲液的pH值和浓度、分离电压以及检测缓冲液的pH值。甲基苯丙胺、3,4-亚甲基二氧基苯丙胺和3,4-亚甲基二氧基甲基苯丙胺的检测限分别为3.3×10⁻⁸ mol/L（0.16 fmol）、1.6×10⁻⁷ mol/L（0.78 fmol）和3.3×10⁻⁸ mol/L（0.16 fmol）。为实际应用，开发了一种用乙酸乙酯进行液液萃取的尿液样本预处理方法，萃取效率高于90%。所建立的同步CE-EC/ECL方法成功应用于尿液样本分析。
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