Liu Yan-Ming, Shi Yan-Mei, Liu Zhuan-Li
Xinyang Normal University, People's Republic of China.
Biomed Chromatogr. 2010 Sep;24(9):941-7. doi: 10.1002/bmc.1389.
A novel and sensitive method for the simultaneous determination of enoxacin and ofloxacin has been established using capillary electrophoresis (CE) coupled with electrochemiluminescence (ECL) detection based on the ECL enhancement of tri(2,2-bipyridyl)ruthenium(II). The conditions for sample solvent type, CE separation and ECL detection were investigated systematically. The analytes were well separated and detected within 7 min. The limits of detection (S/N = 3) of enoxacin and ofloxacin are 9.0 x 10(-9) and 1.6 x 10(-8) mol/L, respectively. The precisions (RSD%) of intraday and interday are less than 2.1 and 4.0%, respectively. The limits of quantitation (S/N = 10) of enoxacin and ofloxacin are 3.2 x 10(-7) and 5.4 x 10(-7) mol/L in human urine samples and 4.1 x 10(-7) and 6.9 x 10(-7) mol/L in human serum samples, respectively. The recoveries of enoxacin and ofloxacin at different concentration levels in human urine, serum and eye drop samples are between 94.0 and 106.7%. The proposed method was successfully applied to the determination of the enoxacin and ofloxacin in human urine, serum and eye drop samples and the monitoring of pharmacokinetics of ofloxacin in human body.
基于三联（2,2 - 联吡啶）钌（II）的电化学发光增强作用，建立了一种新颖且灵敏的同时测定依诺沙星和氧氟沙星的方法，该方法采用毛细管电泳（CE）与电化学发光（ECL）检测联用技术。系统研究了样品溶剂类型、CE分离及ECL检测的条件。分析物在7分钟内实现了良好分离和检测。依诺沙星和氧氟沙星的检测限（S/N = 3）分别为9.0×10⁻⁹和1.6×10⁻⁸ mol/L。日内和日间精密度（RSD%）分别小于2.1%和4.0%。在人尿样中，依诺沙星和氧氟沙星的定量限（S/N = 10）分别为3.2×10⁻⁷和5.4×10⁻⁷ mol/L；在人血清样中，分别为4.1×10⁻⁷和6.9×10⁻⁷ mol/L。依诺沙星和氧氟沙星在人尿、血清及滴眼液样品中不同浓度水平下的回收率在94.0%至106.7%之间。所提方法成功应用于人尿、血清及滴眼液样品中依诺沙星和氧氟沙星的测定以及人体中氧氟沙星的药代动力学监测。
