Rutledge R G, Quellet T, Hattori J, Miki B L
Plant Research Centre, Agriculture Canada, Ottawa.
Mol Gen Genet. 1991 Sep;229(1):31-40. doi: 10.1007/BF00264210.
The Brassica napus rapeseed cultivar Topas contains an acetohydroxyacid synthase (AHAS) multigene family consisting of five members (AHAS 1-5). DNA sequence analysis indicate that AHAS1 and AHAS3 share extensive homology. They probably encode the AHAS enzymes essential for plant growth and development. AHAS2 has diverged significantly from AHAS1 and AHAS3 and has unique features in the coding region of the mature polypeptide, transit peptide and upstream non-coding DNA, which raises the possibility that it has a distinct function. AHAS4 and AHAS5 have interrupted coding regions and may be defective. The complexity of the AHAS multigene family in the allotetraploid species B. napus is much greater than reported for Arabidopsis thaliana and Nicotiana tabacum. Analysis of the presumptive progenitor diploid species B. campestris and B. oleracea indicated that AHAS2, AHAS3 and AHAS4 originate from the A genome, whereas AHAS1 and AHAS5 originate from the C genome. Further variation within each of the AHAS genes in these species was found.
甘蓝型油菜品种托帕斯含有一个由五个成员（AHAS 1 - 5）组成的乙酰羟酸合酶（AHAS）多基因家族。DNA序列分析表明，AHAS1和AHAS3具有广泛的同源性。它们可能编码植物生长和发育所必需的AHAS酶。AHAS2与AHAS1和AHAS3有显著差异，并且在成熟多肽的编码区、转运肽和上游非编码DNA方面具有独特特征，这增加了其具有独特功能的可能性。AHAS4和AHAS5的编码区有中断，可能存在缺陷。异源四倍体物种甘蓝型油菜中AHAS多基因家族的复杂性远高于拟南芥和烟草的报道。对推测的祖先二倍体物种油菜和甘蓝的分析表明，AHAS2、AHAS3和AHAS4起源于A基因组，而AHAS1和AHAS5起源于C基因组。在这些物种的每个AHAS基因中还发现了进一步的变异。
