Bubunenko M G, Gudkov A T
Mol Biol (Mosk). 1991 Jan-Feb;25(1):172-6.
Methods of high-speed centrifugation and limited proteolysis were used to probe the interaction of EF-Tu with EF-Ts on the ribosome. It is shown that EF-Ts dissociates from EF-Tu only after EF-Tu-mediated GTP hydrolysis, i.e. EF-Ts within the EF-Tu.ribosome complexes in the pre-GTP-hydrolysis state co-sediments with the ribosomes and its rate of proteolysis is distinct from that of free EF-Ts. Moreover, as seen from the difference in sensitivity to trypsin of ribosomal proteins L19 and L27 EF-Ts affects the interaction of EF-Tu with the ribosome.
采用高速离心和有限蛋白酶解方法来探究延伸因子Tu（EF-Tu）与延伸因子Ts（EF-Ts）在核糖体上的相互作用。结果表明，EF-Ts仅在EF-Tu介导的GTP水解后才与EF-Tu解离，即在GTP水解前状态的EF-Tu·核糖体复合物中的EF-Ts与核糖体共同沉降，其蛋白酶解速率与游离的EF-Ts不同。此外，从核糖体蛋白L19和L27对胰蛋白酶敏感性的差异可以看出，EF-Ts会影响EF-Tu与核糖体的相互作用。
