Pantin V I, Sats N V, Surin V L, Egorov L V, Solov'ev G Ia, Borovkova T V, Grineva N I
Mol Biol (Mosk). 1991 Jan-Feb;25(1):177-84.
The transport regularity of the [32P]-oligo/polynucleotides and their polyalkylating derivatives into SH2 rat cells transformed with SA7 adenovirus DNA was investigated. Derivatives penetrate the SH2 cells and their distribution in the subcellular fractions are proportional to the concentration of reagent in the medium. The transport efficiency of the derivatives is inhibited sharply with cell concentration increase and practically does not depend on the action of cell metabolism inhibitors. The data obtained assumes the mechanism of the derivatives transport to be liquid endocytosis. Being distributed in the cell components the polyalkylating derivatives were accumulated by the cell nuclei up to 10(5)-10(7) molecules per nucleus. Transport efficiency is much greater in the anchored cells than in the suspended ones. Though essential dephosphorylation of the utilized substances is observed in the SH2 cells, part of them maintain native chain length and the 5'-phosphate group after 1 h incubation in nucleic acids obtained from the cell nuclei.
研究了[32P] - 寡核苷酸/多核苷酸及其多烷基化衍生物进入用SA7腺病毒DNA转化的SH2大鼠细胞的转运规律。衍生物可穿透SH2细胞，其在亚细胞组分中的分布与培养基中试剂的浓度成正比。随着细胞浓度增加，衍生物的转运效率急剧下降，且实际上不依赖于细胞代谢抑制剂的作用。所获得的数据表明衍生物的转运机制为液相内吞作用。多烷基化衍生物分布于细胞成分中，细胞核对其进行积累，每个细胞核可达10(5)-10(7)个分子。固定细胞中的转运效率远高于悬浮细胞。尽管在SH2细胞中观察到所用物质发生了显著的去磷酸化，但在从细胞核获得的核酸中孵育1小时后，其中一部分仍保持天然链长和5'-磷酸基团。
