Rybalkin I N, Chidzhavadze Z G, Florent'ev V L, Bibilashvili R Sh
Mol Biol (Mosk). 1991 Jan-Feb;25(1):223-30.
The highly purified preparations of Bollum's terminal transferase from calf thymus were shown to catalyze, along with the common reaction of nucleotide addition to the 3'-terminus of an oligonucleotide primer, a "non-common" reaction between dNTP or rNTP on one hand, and various alcohols on the other hand. This reaction was carried out with ethylene glycol, glycerol, ethanol and methanol to produce substances containing one molecule of nucleotide, one molecule of alcohol and non-organic pyrophosphate. The reaction conditions are cacodylate buffer, pH 7, 2, in the presence of Mg2+ or Co2+ ions. The structure was determined for the product of the reaction between glycerol and dATP, which appeared to be 2,3-dihydroxypropyl-ether of 2'-deoxyadenosine-5'-monophosphate.
来自小牛胸腺的高度纯化的博勒姆末端转移酶制剂，除了能催化在寡核苷酸引物3'末端添加核苷酸的常见反应外，还能催化一方面是dNTP或rNTP，另一方面是各种醇之间的“非常见”反应。该反应使用乙二醇、甘油、乙醇和甲醇进行，生成含有一分子核苷酸、一分子醇和无机焦磷酸的物质。反应条件为在Mg2+或Co2+离子存在下，pH 7.2的二甲胂酸盐缓冲液。确定了甘油与dATP反应产物的结构，其似乎是2'-脱氧腺苷-5'-单磷酸的2,3-二羟丙基醚。
