基于荧光能量转移的光纤生物传感器。

Fiber-optic biosensors based on fluorescence energy transfer.

作者信息

Meadows D, Schultz J S

机构信息

Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, U.S.A.

出版信息

Talanta. 1988 Feb;35(2):145-50. doi: 10.1016/0039-9140(88)80053-5.

DOI:10.1016/0039-9140(88)80053-5

PMID:18964483

Abstract

A new optical homogeneous biochemical method for the assay of glucose has been developed, based on fluorescence energy transfer between a glucose analog, dextran labeled with fluorescein isothiocyanate (FITC-dextran), and a glucose-receptor protein, Rhodamine-labeled Concanavalin A (Rh-ConA). When FITC-dextran binds to Rh-ConA in solution, and is light-activated, the FITC label transfers its absorbed energy to the Rhodamine label, which then emits light according to its own characteristic fluorescence spectrum. When glucose is added to this solution, the FITC fluorescence intensity increases as FITC-dextran is released from the Rh-ConA and is replaced by glucose. Thus it is possible to determine glucose concentrations directly from the level of FITC fluorescence.

摘要

一种用于检测葡萄糖的新型光学均相生化方法已被开发出来，该方法基于葡萄糖类似物异硫氰酸荧光素标记的葡聚糖（FITC - 葡聚糖）与葡萄糖受体蛋白罗丹明标记的伴刀豆球蛋白A（Rh - ConA）之间的荧光能量转移。当FITC - 葡聚糖在溶液中与Rh - ConA结合并被光激活时，FITC标记将其吸收的能量转移给罗丹明标记，然后罗丹明标记根据其自身的特征荧光光谱发光。当向该溶液中加入葡萄糖时，随着FITC - 葡聚糖从Rh - ConA上释放并被葡萄糖取代，FITC荧光强度增加。因此，可以直接根据FITC荧光水平测定葡萄糖浓度。