Hinshaw V S, Blank H F
Am J Vet Res. 1977 Jan;38(1):55-7.
Specimens obtained from feline leukemia virus (FeLV)-positive cats were examined for infectious FeLV. Feline leukemia virus was detected by a focus-forming assay and confirmed by florescent antibody. Techniques of sample processing were evaluated and adjusted for optimum detection of FeLV. Low levels of FeLV were detected in 2 of 10 oral samples; however, the majority of these samples (17 of 27 tested) produced cytopathic effects in tissue culture which prevented Fe LV detection. Three of 24 urine samples and 1 of 20 rectal specimens were positive for FeLV. One milk sample contained high levels of FeLV.
对从猫白血病病毒(FeLV)阳性猫身上获取的样本进行了传染性FeLV检测。通过焦点形成试验检测猫白血病病毒,并通过荧光抗体进行确认。对样本处理技术进行了评估和调整,以实现对FeLV的最佳检测。在10份口腔样本中有2份检测到低水平的FeLV;然而,这些样本中的大多数(27份检测样本中的17份)在组织培养中产生了细胞病变效应,从而妨碍了FeLV的检测。24份尿液样本中有3份以及20份直肠样本中有1份的FeLV呈阳性。1份牛奶样本中含有高水平的FeLV。