Stavrianakis I G, Soper C J
School of Pharmacy and Pharmacology, University of Bath, U.K.
Pharmazie. 1991 May;46(5):340-1.
A number of Candida species were selected in order to test their ability to N- or O-demethylate codeine. Complex media and chemically defined media used in our experiments. Transformation products were analysed by reversed phase HPLC and TLC methods. N- and O-demethylation of codeine was performed by C. tropicalis NCYC 997, NCYC 405, NCYC 470 and C. albicans LSHTM 3153. Transformation by C. tropicalis NCYC 997 had better results. N- and O-demethylation occurred in the chemically defined medium. Change in the colour of the transformation mixture, in cultures containing more than 5 mmol.l-1 codeine, was attributed to codeine N-oxide, but its presence was not confirmed by HPLC. N- and O-demethylation of codeine by Candida sp. was compared to that occurred in mammals.
为了测试多种念珠菌对可待因进行N-去甲基化或O-去甲基化的能力，我们选择了若干念珠菌菌株。实验中使用了复合培养基和化学成分确定的培养基。通过反相高效液相色谱法（HPLC）和薄层色谱法（TLC）对转化产物进行分析。热带念珠菌NCYC 997、NCYC 405、NCYC 470和白色念珠菌LSHTM 3153可对可待因进行N-去甲基化和O-去甲基化。热带念珠菌NCYC 997的转化效果更佳。在化学成分确定的培养基中发生了N-去甲基化和O-去甲基化。在含有超过5 mmol·l-1可待因的培养物中，转化混合物颜色的变化归因于可待因N-氧化物，但其存在未通过HPLC得到证实。将念珠菌属对可待因的N-去甲基化和O-去甲基化与哺乳动物体内发生的情况进行了比较。
