Shengjun M, Holcombe J A
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78712, U.S.A.
Talanta. 1991 May;38(5):503-10. doi: 10.1016/0039-9140(91)80171-u.
Unicellular green algae have been utilized to preconcentrate Ni(2+) and Co(2+) ions from sea-water and riverine water samples. Studies have shown that rinsing the algae with 0.12M hydrochloric acid improves the adsorption of nickel and cobalt, and the optimum range of pH of extraction is wide. The maximum extraction efficiencies were 84 and 73% for Ni and Co, respectively, at ng/ml levels. The sea-water matrix and relatively small amounts of many impurities reduce the adsorption efficiency for both nickel and cobalt. The preconcentration is achieved by mixing 6 mg of algae with 50-100 ml of sample, and subsequently isolating the algae by centrifugation. The pellet of algae is then resuspended in 1 ml of 0.08M nitric acid, and analyzed as a slurry by graphite-furnace atomic-absorption spectrometry. The values found for nickel and cobalt in riverine (SLRS-1) and sea-water (CASS-1) standard reference materials are within the limits of certification.
单细胞绿藻已被用于从海水和河水样品中预富集镍离子（Ni(2+)）和钴离子（Co(2+)）。研究表明，用0.12M盐酸冲洗藻类可提高镍和钴的吸附量，且萃取的最佳pH范围较宽。在纳克/毫升水平下，镍和钴的最大萃取效率分别为84%和73%。海水基质和相对少量的多种杂质会降低镍和钴的吸附效率。通过将6毫克藻类与50 - 100毫升样品混合，随后通过离心分离藻类来实现预富集。然后将藻类沉淀重新悬浮于1毫升0.08M硝酸中，并通过石墨炉原子吸收光谱法作为悬浮液进行分析。在河流（SLRS - 1）和海水（CASS - 1）标准参考物质中测得的镍和钴值在认证范围内。
