Fernández P, Conde C P, Gutiérrez A, Cámara C
Departamento de Química Analítica, Facultad de Ciencias Químicas de la Universidad Complutense, 28040 Madrid, Spain.
Talanta. 1991 Dec;38(12):1387-92. doi: 10.1016/0039-9140(91)80285-8.
A new, sensitive and rapid spectrofluorimetric flow-injection method, is presented for the determination of trace levels of aluminium based on the formation of a fluorescent complex between aluminium and 5,7-dibromo-8-quinolinol (DBQ) and its extraction into diethylether (lambda(exc) = 400 nm, lambda(em) = 525 nm). Experimental conditions such as pH, reagent concentration, flow-rates, sample volume, extraction coil length, etc., have been optimized for on-line and batch procedures. The detection limits are 1 ppb and 0.3 ppb for batch and on-line systems respectively. The coeflicient of variation is 3.0% at the 4 ppb level for the FIA system. To remove interferences and to preconcentrate aluminium, a chelating resin microcolumn which was able to selectively complex A1(III) and was obtained by immobilizing Chromotrope 2B on AG1-X8 ion-exchange resin, was incorporated into the FIA system. The proposed method was successfully applied to determine aluminium in tap water, food samples and dialysis solutions.
本文提出了一种新的、灵敏且快速的荧光分光光度流动注射法，用于测定痕量铝。该方法基于铝与5,7-二溴-8-喹啉醇（DBQ）形成荧光络合物，并将其萃取到乙醚中（激发波长λ(exc)=400nm，发射波长λ(em)=525nm）。对pH值、试剂浓度、流速、样品体积、萃取盘管长度等实验条件进行了优化，以适用于在线和批量分析流程。批量和在线系统的检测限分别为1ppb和0.3ppb。在4ppb水平下，流动注射分析系统的变异系数为3.0%。为了消除干扰并对铝进行预富集，将一种螯合树脂微柱引入流动注射分析系统，该微柱通过将变色酸2B固定在AG1-X8离子交换树脂上制得，能够选择性地络合Al(III)。所提出的方法成功应用于自来水、食品样品和透析液中铝的测定。
