Department of Analytical Chemistry, Faculty of Marine and Environmental Sciences. University of Cádiz, Puerto Real, Cádiz 11510, Spain.
Talanta. 2003 Jun 13;60(2-3):425-31. doi: 10.1016/S0039-9140(03)00096-1.
A simple reverse flow-injection (rFIA) manifold for the direct determination of aluminum in drinking water is proposed. This rapid and sensitive method is based on the formation of an Al(3+) complex with salicylaldehyde picolinoylhydrazone (SAPH), which shows a maximum blue-green fluorescence (lambda(ex)=384 nm, lambda(em)= 468 nm) at pH 5.4. Operative conditions both for batch and rFIA procedures were investigated including reagent concentration, buffer solutions, injection loop, reacting coil and wavelengths used for the fluorimetric detection. The tolerance limits of foreign ions have been also evaluated, before and after the addition of masking agents. The reverse flow-injection procedure allows determination of Al(3+) at ppb level (LOD: 1.9 mug l(-1)) within a working range of 5-30 mug l(-1). The proposed method was successfully employed for the determination of Al(3+) in several commercial drinking, soft drinking (as certified reference material), and tap water samples.
提出了一种用于直接测定饮用水中铝的简单反向流动注射（rFIA）流路。该快速灵敏的方法基于与水杨醛吡啶甲酰腙（SAPH）形成 Al（3+）配合物，在 pH 5.4 时显示出最大的蓝绿色荧光（λ（ex）= 384nm，λ（em）= 468nm）。对批处理和 rFIA 过程的操作条件（包括试剂浓度、缓冲溶液、进样环、反应盘管和用于荧光检测的波长）进行了研究。还评估了加入掩蔽剂前后外来离子的容忍极限。反向流动注射程序允许在 5-30μg/L 的工作范围内测定 ppb 级（LOD：1.9μg/L）的 Al（3+）。该方法成功地用于测定几种商业饮用水、软饮料（作为认证参考材料）和自来水样品中的 Al（3+）。
