Boyer A E, Devanathan S, Hamilton D, Patonay G
Department of Chemistry, Georgia State University, Atlanta, GA 30303, U.S.A.
Talanta. 1992 May;39(5):505-10. doi: 10.1016/0039-9140(92)80172-a.
Near-infrared dyes can be derivatized with appropriate functional groups for use as analytical probes for numerous applications. A dye derivatized with pH-sensitive functional groups may show spectral changes when the pH of its environment is changed. These dyes are valuable to the researcher since they absorb and fluoresce at long wavelengths where interference is minimal and their absorption maxima permit the use of semiconductor lasers. In this paper we have evaluated the sensitivity to pH of a bis-carboxylic acid derivative of a near infrared dye to illustrate its potential as a probe for determining pH. The dye has an absorption maximum at 795 nm in aqueous solution, a region where several commercially available laser diodes have their output maximum. The analytical utility of near-infrared laser diodes for pH determination has also been evaluated.
近红外染料可以用适当的官能团进行衍生化，以用作多种应用的分析探针。用对pH敏感的官能团衍生化的染料，当其环境的pH发生变化时可能会出现光谱变化。这些染料对研究人员很有价值，因为它们在干扰最小的长波长处吸收和发出荧光，并且它们的最大吸收允许使用半导体激光器。在本文中，我们评估了一种近红外染料的双羧酸衍生物对pH的敏感性，以说明其作为测定pH探针的潜力。该染料在水溶液中的最大吸收波长为795 nm，这是几个市售激光二极管输出最大值的区域。还评估了近红外激光二极管用于pH测定的分析实用性。
