McKelvie I D, Hart B T, Cardwell T J, Cattrall R W
Water Studies Centre and Chemistry Department, Monash University, Caulfield East 3145, Victoria, Australia.
Talanta. 1993 Dec;40(12):1981-93. doi: 10.1016/0039-9140(93)80124-a.
A study of the factors affecting separation and detection of dissolved organic and inorganic phosphorus species found in waters sediments is reported. The system involved the use of gel filtration and flow injection analysis (FIA). Orthophosphate and myo-inositol hexakisphosphate, as model solutes representative of low molecular weight P (LMWP) and high molecular weight P (HMWP), were separated on a Sephadex G25 column incorporated into a flow-injection manifold which utilized photo-oxidation and spectrophotometry for detection of dissolved reactive phosphorus (DRP) and dissolved organic phosphorus (DOP). The influence of eluent pH and ionic strength on adsorption and anionic exclusion of the model solutes is described, and the optimum eluent composition and sample size are described. The method was used to determine LMWP and HMWP in natural and waste waters, and in sediment extracts. Potential limitations of this approach are discussed.
本文报道了一项关于影响水体沉积物中溶解有机磷和无机磷物种分离与检测因素的研究。该系统采用了凝胶过滤和流动注射分析（FIA）。以正磷酸盐和肌醇六磷酸作为代表低分子量磷（LMWP）和高分子量磷（HMWP）的模型溶质，在并入流动注射歧管的葡聚糖凝胶G25柱上进行分离，该歧管利用光氧化和分光光度法检测溶解活性磷（DRP）和溶解有机磷（DOP）。描述了洗脱液pH值和离子强度对模型溶质吸附和阴离子排斥的影响，并阐述了最佳洗脱液组成和进样量。该方法用于测定天然水、废水以及沉积物提取物中的LMWP和HMWP。讨论了该方法的潜在局限性。
