Peat D M, McKelvie I D, Matthews G P, Haygarth P M, Worsfold P J
Department of Environmental Sciences, Plymouth Environmental Research Centre, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK.
Talanta. 1997 Dec 12;45(1):47-55. doi: 10.1016/s0039-9140(97)00105-7.
A rapid method suitable for the determination of dissolved organic phosphorus (DOP) in soil leachates and runoff waters is presented. The flow injection (FI) manifold contains an in-line PTFE reaction coil wrapped around a low power UV lamp and is based on the spectrophotometric determination of dissolved reactive phosphorus (DRP) and mineralised DOP at 690 nm after reduction of phosphomolybdate to molybdenum blue with tin(II) chloride. The linear range was 0-1.5 mg 1(-1) PO(4)-P, with a detection limit (3 s) of 7 mug 1(-1) and a sample throughput of 40 h(-1). Tolerance to potential matrix interferences in soil pore waters, particularly Al(III), Si(IV), Fe(II) and Fe(III), was achieved using a combination of on-line sample pre-treatment by a strong acid ion exchange column, low photoreactor pH and acid induced control of the kinetics of the molybdenum blue reaction. The results obtained with this manifold were in good agreement with those obtained by a batch spectrophotometric reference method.
本文提出了一种适用于测定土壤渗滤液和径流水中溶解有机磷（DOP）的快速方法。流动注射（FI）系统包含一个缠绕在低功率紫外灯周围的在线聚四氟乙烯反应盘管，该系统基于在690nm处分光光度法测定溶解态活性磷（DRP）和矿化的DOP，测定前用氯化亚锡将磷钼酸盐还原为钼蓝。线性范围为0 - 1.5mg 1(-1) PO(4)-P，检测限（3s）为7μg 1(-1)，样品通量为40 h(-1)。通过强酸离子交换柱在线样品预处理、低光反应器pH值以及酸诱导控制钼蓝反应动力学相结合的方法，实现了对土壤孔隙水中潜在基质干扰（特别是Al(III)、Si(IV)、Fe(II)和Fe(III)）的耐受性。该系统获得的结果与分批分光光度参考方法获得的结果高度一致。
