Khalaf K D, Hasan B A, Morales-Rubio A, de la Guardia M
Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
Talanta. 1994 Apr;41(4):547-56. doi: 10.1016/0039-9140(93)e0023-7.
Based on the reaction with p-aminophenol, a series of procedures have been developed for the spectrophotometric determination of phenol and resorcinol. Three basic approaches have been studied: (i) a batch procedure, using the dissolved oxygen as oxidant, (ii) a stopped-flow procedure by use of KIO(4) as oxidant and (iii) a flow injection (FI) procedure developed in the presence of KIO(4). Phenol can be accurately determined at 626 nm, in the stopped-flow mode, after a reaction time of 45 min in 0.005M NaOH, 0.004M KIO(4) and 500 mug/ml of PAP. The development of a manifold, which incorporates a sample parking, is a convenient approach which makes it possible to measure, in the stopped-flow mode, four solutions in one hour. The limit of detection of this method corresponds to 64 ng/ml of phenol Resorcinol can be determined by FI at 540 nm in 0.006M NaOH, 0.0002M KIO(4) and 50 mug/ml PAP with a limit of detection of 6.6 ng/ml and a sample throughput of 300 injections per hour. A combination between the FI procedure for the determination of resorcinol and the stopped-flow procedure for phenol determination provides accurate results in the analysis of spiked samples containing both phenol and resorcinol.
基于与对氨基酚的反应，已开发出一系列用于分光光度法测定苯酚和间苯二酚的程序。研究了三种基本方法：（i）使用溶解氧作为氧化剂的分批程序，（ii）使用高碘酸钾（KIO₄）作为氧化剂的停流程序，以及（iii）在高碘酸钾存在下开发的流动注射（FI）程序。在0.005M氢氧化钠、0.004M高碘酸钾和500μg/ml对氨基酚（PAP）中反应45分钟后，在停流模式下于626nm处可准确测定苯酚。开发一种包含样品停放功能的流路是一种便捷的方法，使得在停流模式下一小时内能够测量四种溶液。该方法的检测限相当于64ng/ml苯酚。间苯二酚可通过流动注射在0.006M氢氧化钠、0.0002M高碘酸钾和50μg/ml对氨基酚中于540nm处测定，检测限为6.6ng/ml，样品通量为每小时300次进样。用于测定间苯二酚的流动注射程序与用于测定苯酚的停流程序相结合，在分析同时含有苯酚和间苯二酚的加标样品时可提供准确结果。
