用于荧光分光光度法测定叶绿素a的均相液-液萃取法
Homogeneous liquid-liquid extraction method for spectrofluorimetric determination of chlorophyll a.
作者信息
Sudo T, Igarashi S
出版信息
Talanta. 1996 Feb;43(2):233-7. doi: 10.1016/0039-9140(95)01748-8.
A new homogeneous liquid-liquid extraction using a fluorocarbon ionic surfactant, Zonyl FSA (FSA), having a diethylthioether group as a spacer between the perfluoroalkyl group and carboxyl group has been developed. In this FSA method, the phase separation phenomena were observed at mild pH (below pH 6). Moreover, by using this extraction method as a preconcentration, a highly sensitive spectrofluorometric determination of chlorophyll a was established. The results for the standard chlorophyll a were as follows. The concentration factor (V(w)/V(o)) was 200 (water phase, V(w), 20 cm(3), water-immiscible phase, V(o), 100 mul), the distribution ratio, log D, was 4.85, the extraction percentage, E, was 99.7%, and the procedure time was approximately 30 min. The calibration curve was linear in the concentration range 2 x 10(-11)-3 x 10(-7) mol dm(-3) and the detection limit (S/N=3) was 1 x 10(-11) mol dm(-3). The relative standard deviation was 0.72% for 10(-8) mol dm(-3) (five determinations).
已开发出一种新型均相液 - 液萃取方法，该方法使用一种氟碳离子表面活性剂全氟辛烷磺酸二乙硫醚（Zonyl FSA，简称FSA），其中二乙硫醚基团作为全氟烷基和羧基之间的间隔基。在这种FSA方法中，在温和的pH值（低于pH 6）下观察到相分离现象。此外，通过将这种萃取方法用作预浓缩手段，建立了一种高灵敏度的叶绿素a荧光分光光度测定法。标准叶绿素a的测定结果如下。浓缩因子（V(w)/V(o)）为200（水相V(w)为20 cm³，与水不混溶相V(o)为100 μl），分配比log D为4.85，萃取率E为99.7%，操作时间约为30分钟。校准曲线在2×10⁻¹¹ - 3×10⁻⁷ mol dm⁻³的浓度范围内呈线性，检测限（S/N = 3）为1×10⁻¹¹ mol dm⁻³。对于10⁻⁸ mol dm⁻³（五次测定），相对标准偏差为0.72%。
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