Agrawal O, Das J V, Gupta V K
School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur 492010 (M.P.), India.
Talanta. 1998 Aug;46(4):501-5. doi: 10.1016/s0039-9140(97)00314-7.
A simple and sensitive method for the determination of isoproturon, a widely used herbicide is described here which is based on alkaline hydrolysis of isoproturon to its corresponding primary amine followed by diazotization and coupling with p-aminoacetophenone in alkaline medium. The absorption maxima of the coloured compound formed is measured at 525 nm. Beer's law is obeyed over the concentration range of 0.5-5.0 mug in a final solution volume of 25 ml (0.02-0.2 ppm). The molar absorptivity and Sandell's sensitivity were found to be 8.6x10(5) l mol(-1) cm(-1) and 0.0002 mug cm(-2), respectively. The method has been satisfactorily applied to the determination of isoproturon in environmental and biological samples.
本文描述了一种简单灵敏的方法来测定异丙隆，一种广泛使用的除草剂。该方法基于异丙隆在碱性条件下水解为相应的伯胺，随后进行重氮化并在碱性介质中与对氨基苯乙酮偶联。形成的有色化合物的最大吸收波长在525nm处测定。在最终溶液体积为25ml（0.02 - 0.2ppm）的情况下，0.5 - 5.0μg的浓度范围内符合比尔定律。摩尔吸光系数和桑德尔灵敏度分别为8.6×10⁵ l mol⁻¹ cm⁻¹和0.0002μg cm⁻²。该方法已成功应用于环境和生物样品中异丙隆的测定。
