Amlathe S, Gupta V K
Department of Chemistry, Ravishankar University, Raipur, India.
Analyst. 1990 Oct;115(10):1385-7. doi: 10.1039/an9901501385.
A sensitive method is described for the spectrophotometric determination of acetone in water. Acetone reacts with vanillin in alkaline medium forming a yellow-orange dye with an absorption maximum at 430 nm. Beer's law is obeyed in the range from 0.5 to 5 p.p.m. of acetone in water. Molar absorptivity and Sandell's sensitivity were found to be 11.0 x 10(3) +/- 100 l mol-1 cm-1 and 0.005 micrograms cm-2, respectively. The important parameters for complete colour development were optimised and the method was used for the determination of acetone in biological samples. The method was also applied successfully to the determination of acetone in air after absorption in sodium hydrogen sulphite solution.
描述了一种用于分光光度法测定水中丙酮的灵敏方法。丙酮在碱性介质中与香草醛反应，形成一种在430nm处有最大吸收的橙黄色染料。水中丙酮含量在0.5至5ppm范围内符合比尔定律。摩尔吸光率和桑德尔灵敏度分别为11.0×10³±100 l·mol⁻¹·cm⁻¹和0.005μg·cm⁻²。优化了显色完全的重要参数，并将该方法用于生物样品中丙酮的测定。该方法还成功应用于亚硫酸氢钠溶液吸收空气中丙酮后的测定。
