Hou S F, Yang K S, Fang H Q, Chen H Y
Department of Chemistry, the State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, PR China.
Talanta. 1998 Nov;47(3):561-7. doi: 10.1016/s0039-9140(98)00081-2.
A novel and robust amperometric enzyme electrode for the determination of glucose was constructed by immobilizing glucose oxidase (GOD) and Os(bpy)(2)Cl-poly(4-vinyl)pyridine (Os-PVP) complex multilayers on thiol self-assembled monolayers surface. The apparent Michaelis-Menton constant K(m)' increased with increasing the number of Os-PVP/GOD multilayers. The concentration range of linear response and detection limit were 0.1-10 and 0.05 mM, the interference of ascorbic acid and uric acid were eliminated by the presence of SAMs and the enzyme electrodes were stable over 3 weeks. The preparation technique may be useful for controlling the performance of multilayer enzyme electrodes by changing the enzyme content.
通过将葡萄糖氧化酶（GOD）和Os(bpy)(2)Cl-聚（4-乙烯基）吡啶（Os-PVP）复合物多层膜固定在硫醇自组装单分子层表面，构建了一种用于测定葡萄糖的新型且稳健的安培型酶电极。表观米氏常数K(m)' 随着Os-PVP/GOD多层膜层数的增加而增大。线性响应的浓度范围和检测限分别为0.1 - 10 mM和0.05 mM，硫醇自组装单分子层的存在消除了抗坏血酸和尿酸的干扰，并且酶电极在3周内保持稳定。该制备技术可能有助于通过改变酶含量来控制多层酶电极的性能。
