Zhou Y, Nagaoka T, Li F, Zhu G
Department of Applied Chemistry, Faculty of Engineering, Yamaguchi University, 2557 Tokiwadai, Ube, 755-8611, Japan.
Talanta. 1999 Feb;48(2):461-7. doi: 10.1016/s0039-9140(98)00267-7.
A novel chemiluminescence (CL) system was evaluated for the determination of hydrogen peroxide, glucose and ascorbic acid based on hydrogen peroxide, which has a catalytic-cooxidative effect on the oxidation of luminol by KIO(4). Hydrogen peroxide can be directly determined by luminol-KIO(4)-H(2)O(2) CL system. The detection limit was 3.0x10(-8) mol l(-1) and the calibration graph was linear over the range of 2.0x10(-7)-6.0x10(-4) mol l(-1). The relative standard deviation of H(2)O(2) was 1.1% for 2.0x10(-6) mol l(-1) (N=11). Glucose was indirectly determined through measuring the H(2)O(2) generated by the oxidation of glucose in the presence of glucose oxidase at pH 7.6. The present method provides a source for H(2)O(2), which, in turn, coupled with the luminol-KIO(4)-H(2)O(2) CL reaction system. The CL was linearly correlated with glucose concentration of 0.6-110 mug ml(-1). The relative standard deviation was 2.1% for 10 mug ml(-1) (N=11). Detection limit of glucose was 0.08 mug ml(-1). Ascorbic acid was also indirectly determined by the suppression of luminol-KIO(4)-H(2)O(2) CL system. The calibration curve was linear over the range of 1.0x10(-7)-1.0x10(-5) mol l(-1) of ascorbic acid. The relative standard deviation was 1.0% for 8.0x10(-7) mol l(-1) (N=11). Detection limit of ascorbic acid was 6.0x10(-8) mol l(-1). These proposed methods have been applied to determine glucose, ascorbic acid in tablets and injection.
基于过氧化氢对高碘酸钾氧化鲁米诺具有催化共氧化作用，对一种用于测定过氧化氢、葡萄糖和抗坏血酸的新型化学发光（CL）体系进行了评估。过氧化氢可通过鲁米诺 - 高碘酸钾 - 过氧化氢CL体系直接测定。检测限为3.0×10⁻⁸ mol l⁻¹，校准曲线在2.0×10⁻⁷ - 6.0×10⁻⁴ mol l⁻¹范围内呈线性。对于2.0×10⁻⁶ mol l⁻¹的过氧化氢，相对标准偏差为1.1%（N = 11）。葡萄糖通过在pH 7.6条件下，在葡萄糖氧化酶存在时测量葡萄糖氧化产生的过氧化氢间接测定。本方法提供了过氧化氢的来源，进而与鲁米诺 - 高碘酸钾 - 过氧化氢CL反应体系相结合。化学发光与葡萄糖浓度在0.6 - 110 μg ml⁻¹范围内呈线性相关。对于10 μg ml⁻¹的葡萄糖，相对标准偏差为2.1%（N = 11）。葡萄糖的检测限为0.08 μg ml⁻¹。抗坏血酸也通过抑制鲁米诺 - 高碘酸钾 - 过氧化氢CL体系间接测定。校准曲线在抗坏血酸浓度为1.0×10⁻⁷ - 1.0×10⁻⁵ mol l⁻¹范围内呈线性。对于8.0×10⁻⁷ mol l⁻¹的抗坏血酸，相对标准偏差为1.0%（N = 11）。抗坏血酸的检测限为6.0×10⁻⁸ mol l⁻¹。这些所提出的方法已应用于片剂和注射液中葡萄糖、抗坏血酸的测定。
