Daly D J, O'Sullivan C K, Guilbault G G
Laboratory of Sensor Development, Department of Chemistry, University College Cork, Cork, Ireland.
Talanta. 1999 Jul;49(3):667-78. doi: 10.1016/s0039-9140(99)00068-5.
One of the most important parameters to be considered when developing a biosensor based on the use of oxidase enzymes is the selective determination of H(2)O(2) in the presence of easily oxidisable interferents. In this work one approach was taken to overcome this problem-the use of metallised carbon electrodes in conjunction with polymers. Polymers, both conducting and nonconducting, have recently become very interesting materials for the suppression of interferences. They are easy to grow on any electrode surface and the extensive range of polymers available provides a huge scope for the large variety of sensors that exist today. They can be grown in organic or aqueous media. In this work, three polymers (polypyrrole, polyaniline and 1,3-diaminobenzene) were examined for their interferent-preventing potential on several types of electrodes. Previous work carried out at the Laboratory of Sensor Development has shown the co-deposition of ruthenium and rhodium on carbon to provide an electrode surface which is highly catalytic and selective towards H(2)O(2)[1]. The co-deposition of Ru, Rh and Pt, as well as Pt on Ru-Rh electrodes was investigated and all these transducers were coupled with the use of the polymers for enhanced elimination of interferences with highly promising results obtained. The best system was seen to be a Ru-Rh metallised electrode polymerised with poly(1,3-diaminobenzene). At an applied potential of +100 mV the response to H(2)O(2) was approximately 200 times greater than the response of any of the potential interferences.
在开发基于氧化酶的生物传感器时，需要考虑的最重要参数之一是在易氧化干扰物存在的情况下对过氧化氢（H₂O₂）进行选择性测定。在这项工作中，采取了一种方法来克服这个问题，即在使用聚合物的同时使用金属化碳电极。聚合物，包括导电和非导电聚合物，最近已成为抑制干扰的非常有趣的材料。它们易于在任何电极表面生长，现有的大量聚合物为当今存在的各种传感器提供了广阔的空间。它们可以在有机或水性介质中生长。在这项工作中，研究了三种聚合物（聚吡咯、聚苯胺和1,3-二氨基苯）在几种类型电极上的抗干扰潜力。传感器开发实验室之前进行的工作表明，钌和铑在碳上的共沉积可提供对H₂O₂具有高催化性和选择性的电极表面[1]。研究了Ru、Rh和Pt的共沉积，以及Pt在Ru-Rh电极上的沉积，并且所有这些换能器都与聚合物结合使用，以增强对干扰的消除，获得了非常有前景的结果。最佳系统是用聚（1,3-二氨基苯）聚合的Ru-Rh金属化电极。在 +100 mV 的施加电位下，对H₂O₂的响应大约比对任何潜在干扰物的响应大200倍。
