Peyrat-Maillard M N, Bonnely S, Berset C
ENSIA, Département Science de l'Aliment, Laboratoire de Chimie des Substances Naturelles: Antioxydants, Arômes, Colorants, 1 avenue des Olympiades, 91744, Massy Cedex, France.
Talanta. 2000 Apr 3;51(4):709-16. doi: 10.1016/s0039-9140(99)00331-8.
The combination of reversed-phase high-performance liquid chromatography with coulometric detection allows the detection of phenolic antioxidants in complex matrices like plant extracts with a high degree of sensitivity and selectivity. According to their voltammetric behaviour, phenolic acids and tocopherols show maximal detector response at low potentials (100-450 mV) while flavonoids show optimal response at two different potential values (one at 0-300 mV and one at 600-900 mV). The potential corresponding to maximal detector response (MDRP) of phenolic acids was shown to be inversely proportional to their antioxidant efficiency as determined in a lipidic model system under strong oxidizing conditions (110 degrees C, intensive oxygenation) or by the DPPH() test. However, such a relationship was not observed for flavonoids.
反相高效液相色谱与库仑检测相结合，能够高度灵敏且选择性地检测复杂基质（如植物提取物）中的酚类抗氧化剂。根据其伏安行为，酚酸和生育酚在低电位（100 - 450 mV）时显示出最大的检测器响应，而黄酮类化合物在两个不同的电位值（一个在0 - 300 mV，另一个在600 - 900 mV）时显示出最佳响应。在强氧化条件（110℃，强烈氧化）下的脂质模型系统中或通过DPPH（）测试确定，酚酸的最大检测器响应电位（MDRP）与其抗氧化效率成反比。然而，黄酮类化合物并未观察到这种关系。
