流动注射分析法结合奎宁敏化的铈（IV）化学发光法测定2-巯基乙烷磺酸钠

Chemiluminescence determination of sodium 2-mercaptoethane sulfonate by flow injection analysis using cerium(IV) sensitized by quinine.

作者信息

Capitán-Vallvey L F, Valencia Mirón M C, Acosta Acosta R

机构信息

Department of Analytical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, Spain.

出版信息

Talanta. 2000 May 5;51(6):1155-61.

PMID:18967946

Abstract

A flow injection method with chemiluminescence (CL) detection is proposed for the determination of sodium 2-mercaptoethane sulfonate (MESNA), a drug often used to reduce the urotoxic effects of antineoplastic alkylating agents. The procedure is based on the reaction of the thiol with Ce(IV) in a sulfuric acid medium and measurement of the CL intensity produced by quinine used as a sensitizer. The optimum conditions for CL emission were investigated. Using the CL peak height as the analytical parameter, MESNA was determined over the concentration range 0.29-2.21 ng (1.97-9.85 mug.l(-1)) with a detection limit of 0.21 ng (1.38 mug.l(-1)) and a relative standard deviation (R.S.D.) of 4.1%. The method was applied satisfactorily to the determination of MESNA in pharmaceutical preparations with percentages of recovery between 94 and 105.

摘要

提出了一种采用化学发光（CL）检测的流动注射法，用于测定2-巯基乙烷磺酸钠（MESNA），这是一种常用于减轻抗肿瘤烷基化剂尿路毒性作用的药物。该方法基于硫醇在硫酸介质中与Ce(IV)的反应，并通过测量用作敏化剂的奎宁产生的化学发光强度来进行测定。研究了化学发光发射的最佳条件。以化学发光峰值高度作为分析参数，在0.29 - 2.21 ng（1.97 - 9.85 μg·L⁻¹）的浓度范围内测定了MESNA，检测限为0.21 ng（1.38 μg·L⁻¹），相对标准偏差（R.S.D.）为4.1%。该方法成功应用于药物制剂中MESNA的测定，回收率在94%至105%之间。