Gong Q J, Qiao J L, Du L M, Dong C, Jin W J
Department of Chemistry, Shanxi University, Taiyuan 030006, People's Republic of China; Yuncheng Advanced Training College, Yuncheng 04000, Shanxi, People's Republic of China.
Talanta. 2000 Nov 1;53(2):359-65. doi: 10.1016/s0039-9140(00)00503-8.
Under controlling pH 3, R-(+)- and S-(-)-ofloxacin (OFLX) enantiomers can be well recognized and resolved by the synchronization-1st derivative fluorescence spectroscopic techniques, and the interference from urine blank also can be eliminated. The linear dynamic ranges are 0.36-2.16 (R), 0.36-2.89 and 3.16-31.6 mug/ml (S), respectively, for determining OFLX in urine samples. The limits of detection are 0.36 mug/ml (R) and the recoveries of R-(+)- and S-(-)-OFLX in urine samples are 97-104%. Relative standard deviation is <6.6%. Pharmacokinetic study of OFLX and levofloxacin shows that R-(+)- and S-(-)-ofloxacin reach their peak concentration in urine samples after a healthy subject has taken tablets for approximately 3 and 6 h, respectively. R-(+)-OFLX can be obviously detected in 5-6 h after a healthy subject has taken tablets, indicating the transformation of S-(-)- to R-(+)-OFLX enantiomer in human body (in vitro).
在pH 3的控制条件下，R-(+)-和S-(-)-氧氟沙星（OFLX）对映体可通过同步一阶导数荧光光谱技术得到很好的识别和分离，同时也能消除尿样空白的干扰。在尿样中测定OFLX时，线性动态范围分别为0.36 - 2.16（R）、0.36 - 2.89和3.16 - 31.6 μg/ml（S）。检测限为0.36 μg/ml（R），尿样中R-(+)-和S-(-)-OFLX的回收率为97 - 104%。相对标准偏差<6.6%。氧氟沙星和左氧氟沙星的药代动力学研究表明，健康受试者服用片剂后，R-(+)-和S-(-)-氧氟沙星分别在约3小时和6小时后在尿样中达到峰值浓度。健康受试者服用片剂后5 - 6小时可明显检测到R-(+)-OFLX，表明人体内（体外）S-(-)-向R-(+)-OFLX对映体的转化。
