Draz Mohammed E, El Wasseef Dalia, El Enany Nahed, Wahba Mary E K
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa 11152, Egypt.
Department of Medicinal Chemistry, Mansoura University, Mansoura 35516, Egypt.
R Soc Open Sci. 2023 Jan 18;10(1):221086. doi: 10.1098/rsos.221086. eCollection 2023 Jan.
First derivative synchronous fluorescence spectroscopy (FDSFS) was applied to detect and quantify either ciprofloxacin (CIP) or levofloxacin (LEV) simultaneously with their photodegradation products, where the photolytic pathway for each analyte was found to be pH dependent. Under the guidance of early published articles, the structure of the produced photolytic products could be concluded, and further related to their resultant fluorescence spectra. The proposed method was subjected to full validation procedure which enables its application in investigating the photodegradation kinetics for both drugs. The obtained kinetic parameters were in accordance with previous reports and could be linked to predict the antibacterial activity of the resultant photodegradation products. These facts prove the suitability of the suggested FDSFS to serve as a stability-indicating assay method and to trace the photofate of CIP and LEV in the ecosystem as potential contaminants. Furthermore, the greenness of the suggested analytical methodology was evaluated via 'Green Analytical Procedure Index' (GAPI), which classifies it as an eco-friendly assay. Eventually, no extraction, treatment or preparation steps were needed during all analysis steps, which renders the proposed assay an appealing tool in environmental analysis.
采用一阶导数同步荧光光谱法（FDSFS）同时检测和定量环丙沙星（CIP）或左氧氟沙星（LEV）及其光降解产物，发现每种分析物的光解途径均依赖于pH值。在早期发表文章的指导下，可以推断出所产生的光解产物的结构，并进一步将其与所得荧光光谱相关联。所提出的方法经过了全面的验证程序，使其能够应用于研究两种药物的光降解动力学。获得的动力学参数与先前的报道一致，并且可以用来预测所得光降解产物的抗菌活性。这些事实证明了所建议的FDSFS适用于作为一种稳定性指示分析方法，并追踪CIP和LEV在生态系统中作为潜在污染物的光命运。此外，通过“绿色分析程序指数”（GAPI）评估了所建议分析方法的绿色度，该指数将其归类为一种环保分析方法。最终，在所有分析步骤中都无需萃取、处理或制备步骤，这使得所提出的分析方法成为环境分析中一种有吸引力的工具。
