Tomčík P, Krajčíková M, Bustin D
Department of Analytical Chemistry, Slovak University of Technology, Radlinského 9, SK-812 37 Bratislava, Slovakia.
Talanta. 2001 Dec 24;55(6):1065-70.
This paper presents a method for the analysis of drugs in dosage form. It is based on galvanostatic generation of oxidation agent from a suitable precursor on one segment of interdigitated microelectrode array (IDA) and its consecutive amperometric detection on second segment. High collection efficiency of this process in comparison to rotational ring disc electrode (RRDE) is a unique feature of IDA system. The transfer of oxidation agent can be influenced by addition of species, which reacts with oxidant. This influence can be used for its determination. Evaluation of generator-collector current dependence (diffusion layer titration curve) reveals the value of generator current I(genE) of the end-point of titration. I(genE) is proportional to the bulk phase concentration of determined species. The method was applied to the analysis of pharmaceuticals Antabus (Disulfiram Alpharma NOR, tetraethylthiuram disulfide (TETD)), a popular drug for alcoholism treatment, and Celaskon (vitamin C, Léciva CZE, ascorbic acid (AA)). From model samples analysis rather low detection limits, 9x10(-7) mol dm(-3), respectively, 4x10(-6) mol dm(-3), were estimated which enables trace content analysis of the drugs. A small size of IDA sensor also makes it suitable for microanalytical improvement.
本文介绍了一种分析剂型中药物的方法。它基于在叉指微电极阵列（IDA）的一段上由合适的前体恒电流产生氧化剂，并在第二段上对其进行连续安培检测。与旋转环盘电极（RRDE）相比，该过程的高收集效率是IDA系统的独特特征。氧化剂的转移可通过添加与氧化剂反应的物质来影响。这种影响可用于其测定。评估发生器-收集器电流依赖性（扩散层滴定曲线）可揭示滴定终点的发生器电流I(genE)值。I(genE)与被测物质的体相浓度成正比。该方法应用于药物安塔布司（戒酒硫，Alpharma NOR公司，二硫化四乙秋兰姆（TETD））和塞拉斯康（维生素C，Léciva CZE公司，抗坏血酸（AA））的分析，这两种药物分别是治疗酒精中毒的常用药物和维生素C。通过对模型样品的分析，估计检测限相当低，分别为9×10⁻⁷ mol dm⁻³和4×10⁻⁶ mol dm⁻³，这使得能够对药物进行痕量含量分析。IDA传感器的小尺寸也使其适用于微分析改进。
