Bartlett Carrie-Ann, Taylor Sarah, Fernandez Carlos, Wanklyn Ceri, Burton Daniel, Enston Emma, Raniczkowska Aleksandra, Black Murdo, Murphy Lindy
Oxtox Limited, Warren House, 5 Mowbray Street, Stockport, SK1 3EJ UK.
Chem Cent J. 2016 Feb 1;10:3. doi: 10.1186/s13065-016-0147-2. eCollection 2016.
Methamphetamine has an adverse effect on the ability to drive safely. Police need to quickly screen potentially impaired drivers therefore a rapid disposable test for methamphetamine is highly desirable. This is the first proof-of-concept report of a disposable electrochemical test for methamphetamine in undiluted saliva.
A screen printed carbon electrode is used for the N,N'-(1,4-phenylene)-dibenzenesulfonamide mediated detection of methamphetamine in saliva buffer and saliva. The oxidized mediator reacts with methamphetamine to give an electrochemically active adduct which can undergo electrochemical reduction. Galvanostatic oxidation in combination with a double square wave reduction technique resulted in detection of methamphetamine in undiluted saliva with a response time of 55 s and lower detection limit of 400 ng/mL.
Using a double square wave voltammetry technique, rapid detection of methamphetamine in undiluted saliva can be achieved, however there is significant donor variation in response and the detection limit is significantly higher than desired. Further optimization of the assay and sensor format is required to improve the detection limit and reduce donor effects.
甲基苯丙胺对安全驾驶能力有不良影响。警方需要快速筛查可能受损的驾驶员，因此非常需要一种用于甲基苯丙胺的快速一次性检测方法。这是关于未稀释唾液中甲基苯丙胺一次性电化学检测的首份概念验证报告。
采用丝网印刷碳电极，用于在唾液缓冲液和唾液中通过N,N'-（1,4-亚苯基）-二苯磺酰胺介导检测甲基苯丙胺。氧化态的媒介物与甲基苯丙胺反应生成一种可进行电化学还原的具有电化学活性的加合物。恒电流氧化结合双平方波还原技术可在未稀释唾液中检测甲基苯丙胺，响应时间为55秒，检测下限为400纳克/毫升。
使用双平方波伏安法技术可实现未稀释唾液中甲基苯丙胺的快速检测，然而不同供体的响应存在显著差异，且检测限明显高于预期。需要进一步优化检测方法和传感器形式，以提高检测限并减少供体效应。
