Xiao Yu-Xiu, Xiao Xiang-Zhu, Feng Yu-Qi, Wang Zhong-Hua, Da Shi-Lu
College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China; Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China.
Talanta. 2002 Apr 8;56(6):1141-51. doi: 10.1016/s0039-9140(01)00667-1.
The high-performance liquid chromatographic behavior of some sulfonamides and quinolones was studied on a p-tert-butyl-calix[6]arene-bonded silica gel stationary phase. The effect of mobile phase variables such as methanol content, ionic strength and pH on their chromatographic behavior was investigated. The retention behavior of sulfonamides on the stationary phase was compared with that on both Zorbax C(18)-bonded silica gel and gamma-(ethylenediamino)propyltriethoxylsilane-bonded silica gel (diamino-bonded phase). The retention mechanism of sulfonamides and quinolones on the stationary phase was also discussed. The results indicate that the stationary phase behaves as a reversed-phase packing and its separation selectivity is much better than that of not only Zorbax C(18) phase but also diamino-bonded phase. Some sulfonamides and quinolones were separated on the stationary phase, but the separation of sulfonamides is far more successful.
研究了一些磺胺类药物和喹诺酮类药物在对叔丁基杯[6]芳烃键合硅胶固定相上的高效液相色谱行为。考察了流动相变量如甲醇含量、离子强度和pH对其色谱行为的影响。将磺胺类药物在固定相上的保留行为与在Zorbax C(18)键合硅胶和γ-(乙二胺基)丙基三乙氧基硅烷键合硅胶（二氨基键合相）上的保留行为进行了比较。还讨论了磺胺类药物和喹诺酮类药物在固定相上的保留机理。结果表明，该固定相表现为反相填料，其分离选择性不仅远优于Zorbax C(18)相，也优于二氨基键合相。一些磺胺类药物和喹诺酮类药物在该固定相上实现了分离，但磺胺类药物的分离更为成功。
