基于共交联双酶体系修饰的 PdIrO(2) 电极的葡萄糖和次黄嘌呤安培传感器。
Amperometric sensor for glucose and hypoxanthine based on a PdIrO(2) modified electrode by a co-crosslinking bienzymic system.
作者信息
Xu Fang, Wang Lin, Gao Mengnan, Jin Litong, Jin Jiye
机构信息
Department of Chemistry, East China Normal University, Shanghai 200062, People's Republic of China.
出版信息
Talanta. 2002 May 16;57(2):365-73. doi: 10.1016/s0039-9140(02)00038-3.
A glassy carbon electrode (GCE) modified with Pd/IrO(2) provides excellent electrocatalytic oxidation of hydrogen peroxide. Glucose oxidase (GOD) and xanthine oxidase (XOD) were co-immobilized on the modified electrode with a thin film Nafion coated on the enzyme layer to form a glucose (Glu)/hypoxanthine (Hx) sensor, without interference from electroactive species such as ascorbic acid (AA) and uric acid (UA). Its response was evaluated with respect to the enzyme amount on the electrode, pH and temperature of the electrolyte. The prepared bienzymic biosensor, used as the detector of HPLC gave a detection limit of 1.0x10(-6) mol l(-1) Glu and 2.0x10(-7) mol l(-1) Hx (Hx) with a linear concentration range of 5.0x10(-6)-2.5x10(-3) mol l(-1) and 1.0x10(-6)-5.0x10(-4) mol l(-1), respectively. Coupled with microdialysis, it was used to monitor the concentrations of Glu and Hx in rat brain.
用 Pd/IrO₂ 修饰的玻碳电极（GCE）对过氧化氢具有优异的电催化氧化性能。葡萄糖氧化酶（GOD）和黄嘌呤氧化酶（XOD）通过在酶层上涂覆一层 Nafion 薄膜共固定在修饰电极上，形成葡萄糖（Glu）/次黄嘌呤（Hx）传感器，不受诸如抗坏血酸（AA）和尿酸（UA）等电活性物质的干扰。针对电极上的酶量、电解质的 pH 值和温度对其响应进行了评估。所制备的双酶生物传感器用作高效液相色谱（HPLC）的检测器时，对 Glu 的检测限为 1.0×10⁻⁶ mol·L⁻¹，对 Hx 的检测限为 2.0×10⁻⁷ mol·L⁻¹，线性浓度范围分别为 5.0×10⁻⁶ - 2.5×10⁻³ mol·L⁻¹ 和 1.0×10⁻⁶ - 5.0×10⁻⁴ mol·L⁻¹。与微透析相结合，用于监测大鼠脑中 Glu 和 Hx 的浓度。
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