在汞电极上对头孢米诺进行电化学还原及其在尿中的伏安法测定。

Electrochemical reduction of cefminox at the mercury electrode and its voltammetric determination in urine.

机构信息

Department of Analytical Chemistry, Faculty of Chemistry, Universidad de Sevilla, Professor Gracia Gonzalez 19, 41012 Seville, Spain.

出版信息

Talanta. 2003 Jan 2;59(1):137-46. doi: 10.1016/s0039-9140(02)00468-x.

DOI:10.1016/s0039-9140(02)00468-x

PMID:18968893

Abstract

The electrochemical behaviour of cefminox in phosphate buffers solutions over pH range 2.0-9.0 using differential-pulse polarography, DC-tast polarography, cyclic voltammetry and linear sweep voltammetry (staircase) has been studied. In acidic media, a non reversible diffusion-controlled reduction involving two electrons occurs and the mechanism for the reduction was suggested. A differential-pulse polarographic method for the determination of cefminox in the concentration range 5.8x10(-6)-6.0x10(-5) M with a detection limit of 1.76x10(-6) M was proposed. Also, a method based on controlled adsorptive pre-concentration of cefminox on the hanging mercury drop electrode followed by linear sweep voltammetry, allows its determination in the concentration range 8.3x10(-8)-1.5x10(-6) M with a detection limit of 2.47x10(-8) M. These methods have been used for the direct determination of cefminox in human urine with recoveries between 98 and 103%, and precision around +/-2%.

摘要

使用差分脉冲极谱法、直流示波极谱法、循环伏安法和线性扫描伏安法（阶跃）研究了头孢米诺在 pH 值 2.0-9.0 的磷酸盐缓冲溶液中的电化学行为。在酸性介质中，发生了一个涉及两个电子的不可逆扩散控制还原，并且提出了还原的机制。提出了一种差分脉冲极谱法，用于测定浓度范围为 5.8x10(-6)-6.0x10(-5) M 的头孢米诺，检测限为 1.76x10(-6) M。此外，基于在悬汞滴电极上吸附预浓缩头孢米诺，然后进行线性扫描伏安法的方法，允许在浓度范围 8.3x10(-8)-1.5x10(-6) M 内测定，检测限为 2.47x10(-8) M。这些方法已用于直接测定人尿中的头孢米诺，回收率在 98%至 103%之间，精密度约为正负 2%。