Department of Medical Research and Education, Veterans General Hospital-Taipei, Taipei, Taiwan, ROC.
Talanta. 2003 Jul 4;60(4):679-85. doi: 10.1016/S0039-9140(03)00142-5.
This paper describes a liquid chromatography-electrospray-ion trap mass spectrometry (LC-ES-ITMS) method for the determination of aristolochic acid I and II (AA-I and AA-II) in medicinal plants and Chinese herbal remedies. A reversed phase C(18) column with gradient elution was utilized. The effects of mobile phase additives, acetic acid and ammonium acetate, on LC separation and ES ionization were investigated. For both AA-I and AA-II, the M+NH(4) ion was found to be the precursor ion for target MS/MS analysis. The MS/MS product ion, M+H-44, was used for the quantitative measurement of AA-I and AA-II. The linearity was good from 0.03 to 5 mug ml(-1) and good correlation (r(2)=0.999) over the range examined was determined for both AA. The detection limit based on a signal-to-noise ratio of three was 0.012 and 0.015 mug ml(-1) for AA-I and AA-II, respectively. Various Chinese herbal remedies obtained from renal failure patients and medicinal plants were examined by this newly developed method.
本文描述了一种液相色谱-电喷雾-离子阱质谱（LC-ES-ITMS）法，用于测定中药材和草药中的马兜铃酸 I 和 II（AA-I 和 AA-II）。采用反相 C(18)柱进行梯度洗脱。考察了流动相添加剂乙酸和乙酸铵对 LC 分离和 ES 电离的影响。对于 AA-I 和 AA-II，均发现M+NH(4)离子是目标 MS/MS 分析的前体离子。MS/MS 产物离子M+H-44用于 AA-I 和 AA-II 的定量测定。在考察的范围内，AA 的线性良好，线性范围为 0.03 至 5 µg ml(-1)，相关性良好（r(2)=0.999）。AA-I 和 AA-II 的检测限分别为 0.012 和 0.015 µg ml(-1)，基于信噪比为三。用新开发的方法对来自肾衰竭患者的各种中草药和药用植物进行了检测。
