Salama Suzy M, AlRashdi Ahmed S, Abdulla Mahmood A, Hassandarvish Pouya, Bilgen Mehmet
Biophysics Department, Faculty of Medicine, Erciyes University, 38039, Kayseri, Turkey.
BMC Complement Altern Med. 2013 Oct 24;13:279. doi: 10.1186/1472-6882-13-279.
Chalcone Panduratin A (PA) has been known for its antioxidant property, but its merits against oxidative damage in liver cells has yet to be investigated. Hence, the paper aimed at accomplishing this task with normal embryonic cell line WRL-68.
PA was isolated from Boesenbergia rotunda rhizomes and its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and ferric reducing power (FRAP) activities were measured in comparison with that of the standard reference drug Silymarin (SI). Oxidative damage was induced by treating the cells with 0.04 g/ml of toxic thioacetamide for 60 minutes followed by treatment with 1, 10 and 100 μg/ml concentrations of either PA or SI. The severities of oxidative stress in the control and experimental groups of cells were measured by Malondialdehyde (MDA) levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities.
PA exhibited an acceptable DPPH scavenging and FRAP activities close to that of Silymarin. Treating the injured cells with PA significantly reduced the MDA level and increased the cell viability, comparable to SI. The activities of SOD, CAT and GPx were significantly elevated in the PA-treated cells in a dose dependent manner and again similar to SI.
Collectively, data suggested that PA has capacity to protect normal liver cells from oxidative damage, most likely via its antioxidant scavenging ability.
查耳酮潘多拉丁A（PA）以其抗氧化特性而闻名，但其对肝细胞氧化损伤的益处尚未得到研究。因此，本文旨在利用正常胚胎细胞系WRL-68完成这项任务。
从圆叶姜根茎中分离出PA，并与标准参考药物水飞蓟素（SI）比较，测定其对2,2-二苯基-1-苦基肼（DPPH）的清除能力和铁还原能力（FRAP）。用0.04 g/ml有毒硫代乙酰胺处理细胞60分钟，然后用1、10和100 μg/ml浓度的PA或SI处理，诱导氧化损伤。通过丙二醛（MDA）水平、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性来测定对照组和实验组细胞氧化应激的严重程度。
PA表现出可接受的DPPH清除能力和接近水飞蓟素的FRAP活性。用PA处理受损细胞可显著降低MDA水平并提高细胞活力，与SI相当。在PA处理的细胞中，SOD、CAT和GPx的活性以剂量依赖的方式显著升高，同样与SI相似。
总体而言，数据表明PA有能力保护正常肝细胞免受氧化损伤，很可能是通过其抗氧化清除能力实现的。
