Alcázar A, Fernández-Cáceres P L, Martı X0301 N M J, Pablos F, González A G
Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, E-41012 Seville, Spain.
Talanta. 2003 Oct 17;61(2):95-101. doi: 10.1016/S0039-9140(03)00244-3.
An ion chromatographic method for the simultaneous determination of organic acids and inorganic ions is described. Acetic, malic, ascorbic, citric, malic and succinic acids, chloride and phosphate were determined in coffee and tea samples. The separation is performed on an anion-exchange column operated at 40 degrees C within 25 min by an isocratic elution with 0.6 mM aqueous potassium hydrogenphthalate (pH 4.0) solution containing 4% (v/v) acetonitrile as eluent and determination by conductivity detection. The method does not need a special sample treatment and was successfully applied to the analysis of black, green and oolong tea samples. Also, green and roasted coffee samples from the varieties arabica and robusta were analyzed.
本文描述了一种同时测定有机酸和无机离子的离子色谱方法。该方法用于测定咖啡和茶样品中的乙酸、苹果酸、抗坏血酸、柠檬酸、苹果酸和琥珀酸、氯离子和磷酸根离子。分离在阴离子交换柱上进行，柱温40℃，采用0.6 mM邻苯二甲酸氢钾水溶液（pH 4.0）含4%（v/v）乙腈作为洗脱剂进行等度洗脱，25分钟内完成分离，并用电导检测法进行测定。该方法无需特殊的样品处理，已成功应用于红茶、绿茶和乌龙茶样品的分析。此外，还对阿拉比卡和罗布斯塔品种的生咖啡和烘焙咖啡样品进行了分析。
