Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, 05508900 São Paulo, SP, Brazil.
Talanta. 2003 Dec 4;61(5):725-32. doi: 10.1016/S0039-9140(03)00334-5.
This paper describes the use of a thin-layered dual-band electrochemical cell operating at flow conditions to determine dipyrone (sodium salt of 1-phenil-2,3-dimethyl-4-methylaminomethanesulfonate-5-pyrazolone) by reaction with electrogenerated iodine. The electrolytic cell consisted of two closely spaced gold electrodes, the upper stream electrode serving as the generator electrode and the downstream electrode working as the collector electrode. A linear dynamic range from 2 to 15 mumol l(-1) dipyrone was obtained by using a sample volume of 100 mul, with a detection limit of 1.1 mumol l(-1). Standard deviation (S.D.) of 3.4% for 20 repetitive injections of a 40 mumol l(-1) dipyrone solution and sampling frequency of 90 h(-1) were achieved. The results obtained with the thin-layered dual-band electrochemical cell for dipyrone determination in three different pharmaceutical samples compared well with those found by iodimetry with coulometrically generated iodine.
本文描述了使用薄层双频电化学池在流动条件下通过与电生成的碘反应来测定二吡咯酮（1-苯基-2,3-二甲基-4-甲氨基甲磺酸钠-5-吡唑酮）。该电解池由两个紧密间隔的金电极组成，上流电极作为发生器电极，下游电极作为收集器电极。通过使用 100μl 的样品体积，获得了二吡咯酮的线性动态范围为 2 至 15μmol l(-1)，检测限为 1.1μmol l(-1)。对于 20 次重复注入 40μmol l(-1)二吡咯酮溶液的标准偏差（S.D.）为 3.4%，采样频率为 90 h(-1)。在三种不同的药物样品中二吡咯酮的测定中，薄层双频电化学池的结果与通过库仑法生成的碘的碘量法得到的结果非常吻合。
