Christersson C E
Department of Prosthetic Dentistry, Faculty of Odontology, Lund University, Malmö, Sweden.
Swed Dent J Suppl. 1991;77:1-82.
A parallel-plate flow cell system was developed to assess individual and combined parameters relevant to oral microbial retention. In this system, internal reflection germanium prisms represented oral solid surfaces including tooth surfaces. The system used, allowed adsorbed protein films to be analyzed in situ by multiple non-destructive surface analytical techniques, without provoking surface associated biofilms. Using a range of biochemical and biophysical techniques, germanium prisms of medium critical surface tension were noted to accurately model film formation at tooth-saliva interfaces. A range of clinically relevant temperatures (22 and 37 degrees C), rinse shear rates (1 and 32 ml/min) and critical surface tensions (high, medium and low) were systematically tested in the flow-cell system for their influence, if any, on salivary microbial retention from whole human saliva. Comparisons of the interfacial organization of adsorbed material were made between human parotid (HPS) and submandibular-sublingual (HSMSL) salivas. The influence of these salivary secretions was also tested on the retention capacities of Streptococcus sanguis and Streptococcus salivarius. Microbial retention was found to be significantly dependent on initial critical surface tensions and rinse flow rates, but not to be affected by tested temperatures. When compared to prisms of low and high critical surface tension, medium critical surface tension prisms retained the highest numbers of microorganisms. No statistically verified morphological selectivity was observed in the retained microbial populations. When the interfacial organizations of HPS and HSMSL were evaluated at low- and medium energy surfaces in the absence and presence the streptococci, comparatively thinner and denser films with pronounced bacterial retention was noted for HPS on prisms of low critical surface tension. In the presence of saliva or salivary fractions, no difference in retention capacities was observed between the tested bacterial strains. The data suggest that in the presence of saliva, physico-chemical and mechanical factors have a major influence on the short term microbial retention to solid surfaces. The amount of salivary proteins being initially retained to a material also seems to be more directly related to the critical surface tension quality of the solid than to the type of bacterial strain present in suspension. The empirical critical surface tension of a material is thus a useful predictor of both microbial retention capacities and primary interactions between saliva and solid surfaces.
开发了一种平行板流动池系统，以评估与口腔微生物滞留相关的个体参数和综合参数。在该系统中，内反射锗棱镜代表包括牙齿表面在内的口腔固体表面。所使用的系统允许通过多种无损表面分析技术对吸附的蛋白质膜进行原位分析，而不会引发与表面相关的生物膜。使用一系列生化和生物物理技术，发现具有中等临界表面张力的锗棱镜能够准确模拟牙齿 - 唾液界面处的膜形成。在流动池系统中系统地测试了一系列临床相关温度（22和37摄氏度）、冲洗剪切速率（1和32毫升/分钟）和临界表面张力（高、中、低）对全人类唾液中唾液微生物滞留的影响（如有）。对人腮腺唾液（HPS）和颌下 - 舌下唾液（HSMSL）中吸附物质的界面组织进行了比较。还测试了这些唾液分泌物对血链球菌和唾液链球菌滞留能力的影响。发现微生物滞留显著依赖于初始临界表面张力和冲洗流速，但不受测试温度的影响。与低临界表面张力和高临界表面张力的棱镜相比，中等临界表面张力的棱镜保留的微生物数量最多。在保留的微生物群体中未观察到经统计学验证的形态选择性。当在不存在和存在链球菌的情况下，在低能和中能表面评估HPS和HSMSL的界面组织时，在低临界表面张力的棱镜上，HPS的膜相对更薄且更致密，细菌滞留明显。在存在唾液或唾液成分的情况下，测试的细菌菌株之间未观察到滞留能力的差异。数据表明，在存在唾液的情况下，物理化学和机械因素对微生物在固体表面的短期滞留具有主要影响。最初保留在材料上的唾液蛋白量似乎也与固体的临界表面张力性质更直接相关，而不是与悬浮液中存在的细菌菌株类型相关。因此，材料的经验临界表面张力是微生物滞留能力以及唾液与固体表面之间初级相互作用的有用预测指标。
