Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli "Federico II", Parco Gussone, Portici (Napoli) 80055, Italy.
Talanta. 2004 Feb 27;62(3):523-30. doi: 10.1016/j.talanta.2003.08.028.
Beauvericin (BEA), a supercritical fluid extraction with supercritical carbon dioxide from maize was investigated. Extraction efficiencies under several different extraction conditions were examined. Pressure, temperature, extraction time, organic modifier and water matrix content (10%) were investigated. The best extraction conditions were at a temperature of 60 degrees C, 3200psi, for 30min static extraction time and methanol as modifier solvent. Extraction recovery of 36% without modifier by adding water to the matrix in the extraction vessel (reproducibility relative standard deviations (R.S.D.)=3-5%) were recorded. Extraction recovery of 76.9% with methanol as co-solvent (reproducibility R.S.D.=3-5%) was obtained. Data shows that SFE gives a lower BEA recovery compared to conventional extraction protocol with organic solvents while SFE with modifier and conventional extraction yields are comparable. BEA extract contents were determined by high pressure liquid chromatography (HPLC) with a diode array detector (DAD) at 205nm and BEA peak confirmed by LC-MS. Acetonitrile-water as mobile phase and column C-18 were both tested. Instrumental and analytical parameters were optimized in the range linear interval from 1 to 500mgkg(-1) and reached a detection limit of 2ng.
从玉米中用超临界二氧化碳进行了伏马菌素（BEA）的超临界流体萃取研究。考察了几种不同萃取条件下的萃取效率。考察了压力、温度、萃取时间、有机改性剂和水基质含量（10%）。最佳萃取条件为温度 60°C，压力 3200psi，静态萃取时间 30min，甲醇为改性剂溶剂。在萃取容器中向基质中加水（萃取回收率为 36%，重现性相对标准偏差（RSD）=3-5%）而不加改性剂时，萃取回收率为 76.9%。当使用甲醇作为共溶剂时（重现性 RSD=3-5%），萃取回收率为 76.9%。数据表明，与有机溶剂的常规萃取方法相比，SFE 对 BEA 的回收率较低，而添加改性剂的 SFE 和常规萃取的产率相当。采用二极管阵列检测器（DAD）在 205nm 处的高效液相色谱法（HPLC）测定 BEA 提取物含量，并通过 LC-MS 确认 BEA 峰。分别测试了乙腈-水作为流动相和 C-18 柱。在 1 至 500mgkg（-1）的线性间隔范围内优化了仪器和分析参数，检测限达到 2ng。
