Yang Lirong, Zhang Xing, Chen Anliang, Li Guangze
Biorational Pesticide Research and Service Center, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China.
Se Pu. 2004 May;22(3):263-6.
An extraction method of tebufenozide using supercritical fluid extraction (SFE) and high performance liquid chromatography (HPLC) was developed. The selected conditions were 48.3 MPa (7000 psi), 60 degrees C, 20 min of static time, dynamic extraction with 10 mL of CO2, 0.04 mL/g of methanol as static modifier, and 5 mL of acetone as collecting solvent. Under these conditions, the recovery of tebufenozide extracted by SFE was 100.75%. The extract was analyzed directly by HPLC with a photodiode array detector. The chromatographic conditions were: UV detection wavelength, 245 nm; column, a C18 column; mobile phase, acetonitrile-water (55:45, v/v); flow rate, 1.0 mL/min; injection volume, 5 microL.
开发了一种使用超临界流体萃取（SFE）和高效液相色谱（HPLC）提取虫酰肼的方法。所选条件为48.3兆帕（7000磅力/平方英寸）、60摄氏度、20分钟静态时间、用10毫升二氧化碳进行动态萃取、0.04毫升/克甲醇作为静态改性剂以及5毫升丙酮作为收集溶剂。在这些条件下，通过SFE萃取的虫酰肼回收率为100.75%。提取物用光电二极管阵列检测器通过HPLC直接分析。色谱条件为：紫外检测波长245纳米；色谱柱，C18柱；流动相，乙腈 - 水（55:45，v/v）；流速，1.0毫升/分钟；进样量，5微升。
