Ocaña J A, Barragán F J, Callejón M
Department of Analytical Chemistry, Faculty of Chemistry, E-41012 Seville, Spain.
Talanta. 2004 Jun 17;63(3):691-7. doi: 10.1016/j.talanta.2003.12.016.
Fluorescence and terbium-sensitised luminescence properties of new quinolone garenoxacin have been studied. The fluorimetric method allows the determination of 0.060-0.600mugml(-1) of garenoxacin in aqueous solution containing HCl/KCl buffer (pH 1.5) with lambda(exc)=282nm and lambda(em)=421nm. Micellar-enhanced fluorescence was also studied, leading to a higher than 400% increase in analytical signal in presence of 12mM sodium dodecyl sulphate (SDS), allowing the determination of 0.020-0.750mugml(-1) of garenoxacin. The terbium-sensitised luminescence method allows the determination of 0.100-1.500mugml(-1) of garenoxacin in 12mM SDS solution containing 0.08M acetic acid/sodium acetate buffer (pH 4.1) and 7.5mM Na(2)SO(3) (chemical deoxygenation agent), with lambda(exc)=281nm and lambda(em)=546nm. Relative standard deviation (R.S.D.) values for the three methods were in the range 1.0-2.0%. The proposed procedures have been applied to the determination of garenoxacin in spiked human urine and serum.
对新型喹诺酮类药物加替沙星的荧光和铽敏化发光特性进行了研究。荧光分析法可在含有HCl/KCl缓冲液（pH 1.5）的水溶液中，于激发波长λ(exc)=282nm和发射波长λ(em)=421nm条件下测定0.060 - 0.600μg/ml的加替沙星。还研究了胶束增敏荧光，在存在12mM十二烷基硫酸钠（SDS）时，分析信号增强超过400%，可测定0.020 - 0.750μg/ml的加替沙星。铽敏化发光法可在含有0.08M乙酸/乙酸钠缓冲液（pH 4.1）和7.5mM亚硫酸钠（化学脱氧剂）的12mM SDS溶液中，于激发波长λ(exc)=281nm和发射波长λ(em)=546nm条件下测定0.100 - 1.500μg/ml的加替沙星。这三种方法的相对标准偏差（R.S.D.）值在1.0 - 2.0%范围内。所提出的方法已应用于加标人尿液和血清中加替沙星的测定。
