Stefánka Zsolt, Abrankó László, Dernovics Mihály, Fodor Péter
Department of Applied Chemistry, Faculty of Food Science, Corvinus University, Villányi út 29- 33, 1118 Budapest, Hungary.
Talanta. 2004 Jun 17;63(3):705-12. doi: 10.1016/j.talanta.2003.12.019.
A flow injection (FI) method was developed using hydraulic high-pressure nebulization as a sample introduction system, coupled to inductively coupled plasma time-of-flight mass spectrometer (ICP-TOFMS) for rapid and simultaneous determination of 19 elements. The operating conditions of the system (analyte flow rate, heating and cooling temperatures of the desolvation module, carrier gas flow rate) for the simultaneous determination of 19 analytes were optimised. The optimum parameters of the sample introduction system were found to be 1.4mlmin(-1) and 1.35lmin(-1) for the analyte solution and nebulizer flow rates, respectively. A compromised condition for heating and cooling stage temperatures of 170 and -5 degrees C was chosen. The detection limits were compared to those obtained by using ICP-TOFMS with alternative sample introduction techniques e.g. conventional nebulization, flow injection chemical hydride generation (FI-CHG) and the obtained results were comparable or better than those resulting from alternative sample introduction. Applying the optimised conditions the simultaneous determination of Ag, As, Ba, Cd, Co, Cu, Ga, In, Li, Mn, Mo, Pb, Sb, Se, Sn, Sr, Tl, V and Zn was carried out. Absolute detection limits (3sigma) in the range of 2-750pg and precision between 0.5 and 9.6% from five replicate measurements of 10ngml(-1) multielemental sample solutions were achieved by using a 200mul sample loop. The developed method was applied for the analysis of certified reference materials of biological origin (TORT-2 "Lobster Hepatopancrease", BCR-422 "Cod Muscle" and IAEA MA-B-3/TM "Fish Homogenate"), and the results showed good agreement with the certified values.
开发了一种流动注射（FI）方法，该方法使用液压高压雾化作为样品引入系统，与电感耦合等离子体飞行时间质谱仪（ICP-TOFMS）联用，用于快速同时测定19种元素。优化了该系统用于同时测定19种分析物的操作条件（分析物流速、去溶剂模块的加热和冷却温度、载气流速）。发现样品引入系统的最佳参数分别为分析物溶液流速1.4mlmin(-1)和雾化器流速1.35lmin(-1)。选择了加热和冷却阶段温度分别为170和-5℃的折衷条件。将检测限与使用ICP-TOFMS结合其他样品引入技术（如传统雾化、流动注射化学氢化物发生（FI-CHG））获得的检测限进行比较，所得结果与其他样品引入方法相当或更好。应用优化条件对Ag、As、Ba、Cd、Co、Cu、Ga、In、Li、Mn、Mo、Pb、Sb、Se、Sn、Sr、Tl、V和Zn进行了同时测定。使用200μl样品环，对10ngml(-1)多元素样品溶液进行五次重复测量，绝对检测限（3σ）在2-750pg范围内，精密度在0.5%-9.6%之间。所开发的方法应用于生物源标准参考物质（TORT-2“龙虾肝胰腺”、BCR-422“鳕鱼肌肉”和IAEA MA-B-3/TM“鱼匀浆”）的分析，结果与认证值显示出良好的一致性。
