Nascentes Clésia C, Arruda Marco A Z, Nogueira Ana Rita A, Nóbrega Joaquim A
Grupo de Análise Instrumental Aplicada, Departamento de Quimica, Universidade Federal de São Carlos, P.O. Box 676, 13560-970 São Carlos, SP, Brazil.
Talanta. 2004 Nov 15;64(4):912-7. doi: 10.1016/j.talanta.2004.04.004.
In the present work, thermospray flame furnace atomic absorption spectrometry (TS-FF-AAS) was employed for Cu and Zn determination in bovine milk and fruit juice samples without any pretreatment. TS-FF-AAS system was optimized and a sample volume of 300mul was injected into the carrier stream (0.014moll(-1) HNO(3) at a flow rate of 0.4mlmin(-1)), and it was introduced into a hot Ni tube. The detection limits obtained for Cu and Zn in aqueous solution were 2.2 and 0.91mugl(-1), respectively, and 3.2mugl(-1) for Cu in a medium containing water-soluble tertiary amines. The relative standard deviations varied from 2.7 to 4.2% (n=12). Sample preparation was carried out by simple dilution in water or in water-soluble tertiary amines medium. Accuracy was checked by performing addition-recovery experiments as well as by using reference materials (whole milk powder, non-fat milk powder, and infant formula). Recoveries varied from 97.7 to 105.3% for Cu and Zn. All results obtained for reference materials were in agreement with certified values at a 95% confidence level.
在本研究中，采用热喷雾火焰炉原子吸收光谱法（TS-FF-AAS）在无需任何预处理的情况下测定牛奶和果汁样品中的铜和锌。对TS-FF-AAS系统进行了优化，将300微升样品注入载流（0.014摩尔每升的硝酸，流速为0.4毫升每分钟），并引入热镍管中。水溶液中铜和锌的检测限分别为2.2和0.91微克每升，在含有水溶性叔胺的介质中铜的检测限为3.2微克每升。相对标准偏差在2.7%至4.2%之间（n = 12）。通过在水或水溶性叔胺介质中简单稀释进行样品制备。通过进行加标回收实验以及使用参考物质（全脂奶粉、脱脂奶粉和婴儿配方奶粉）来检查准确性。铜和锌的回收率在97.7%至105.3%之间。参考物质获得的所有结果在95%置信水平下与认证值一致。
