Rezaei Zahra, Hemmateenejad Bahram, Khabnadideh Soghra, Gorgin Mina
Department of Medicinal Chemistry, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
Talanta. 2005 Jan 15;65(1):21-8. doi: 10.1016/j.talanta.2004.05.008.
Carbamazepine (CBZ) and phenytoin (PHT) are two antiepileptic drugs which are used simultaneously. In this paper a partial least-squares (PLS) calibration method is described for the simultaneous spectrophotometric determination of CBZ and PHT in plasma. Standard binary mixtures of CBZ and PHT have been resolved by application of PLS-1 to their UV spectra. Then, the binary standard solutions, spiked to plasma, were prepared and after the extraction of the drugs, their corresponding UV spectrum were analyzed by PLS regression to calculate the concentration of drugs in unknown plasma. A leave one out cross-validation procedure was employed to find the optimum numbers of latent variables using PRESS. A HPLC method was also applied for simultaneous determination of two drugs in the plasma and in methanol. The mean recoveries obtained by PLS were 98.4 and 98.2 for CBZ and PHT and those obtained by HPLC were 100.1 and 101.7, respectively. Although, the HPLC method showed better performance than PLS, it was found that the results obtained by PLS were comparable with those obtained by HPLC method.
卡马西平（CBZ）和苯妥英（PHT）是两种同时使用的抗癫痫药物。本文描述了一种偏最小二乘法（PLS）校准方法，用于同时分光光度法测定血浆中的CBZ和PHT。通过将PLS-1应用于CBZ和PHT的紫外光谱，解析了它们的标准二元混合物。然后，制备添加到血浆中的二元标准溶液，药物萃取后，通过PLS回归分析其相应的紫外光谱，以计算未知血浆中药物的浓度。采用留一法交叉验证程序，使用预测残差平方和（PRESS）来确定最佳潜变量数。还应用高效液相色谱法（HPLC）同时测定血浆和甲醇中的两种药物。PLS法测得CBZ和PHT的平均回收率分别为98.4%和98.2%，HPLC法测得的平均回收率分别为100.1%和101.7%。虽然HPLC法表现优于PLS法，但发现PLS法得到的结果与HPLC法相当。
