Nejem R'afat Mahmoud, Stefan Raluca-Ioana, Staden Jacobus Frederick van, Aboul-Enein Hassan Y
Department of Chemistry, University of Pretoria, Pretoria 0002, South Africa.
Talanta. 2005 Jan 30;65(2):437-40. doi: 10.1016/j.talanta.2004.06.040.
Quantitative assay of l-2-hydroxyglutaric acid (l-2-HGA) is important for the diagnosis of l-2-hydroxyglutaric aciduria. Three enantioselective, potentiometric membrane electrodes (EPMEs) based on maltodextrins with different dextrose equivalent (DE) (DE: 4.0-7.0 (I), 13.0-17.0 (II), 16.5-19.5 (III)), were designed for the enantioanalysis of l-2-HGA. The enantioselective, potentiometric membrane electrodes can be used reliably for enantiopurity assay of l-2-HGA using the direct potentiometric method in the ranges of 10(-9)-10(-5), 10(-6)-10(-3) and 10(-8)-10(-5)molL(-1) for the enantioselective, potentiometric membrane electrodes based on maltodextrins I, II and III, respectively, with very low detection limits. A high reliability was obtained when the electrodes were used for the assay of l-2-HGA in urine samples.
L-2-羟基戊二酸（L-2-HGA）的定量测定对于L-2-羟基戊二酸尿症的诊断至关重要。基于不同葡萄糖当量（DE）（DE：4.0 - 7.0（I）、13.0 - 17.0（II）、16.5 - 19.5（III））的麦芽糊精设计了三种对映选择性电位膜电极（EPME），用于L-2-HGA的对映体分析。这些对映选择性电位膜电极可分别基于麦芽糊精I、II和III，在10⁻⁹ - 10⁻⁵、10⁻⁶ - 10⁻³和10⁻⁸ - 10⁻⁵molL⁻¹范围内，采用直接电位法可靠地用于L-2-HGA的对映体纯度测定，检测限极低。当这些电极用于尿液样本中L-2-HGA的测定时，获得了高可靠性。
