Fernández-Torres Rut, Pérez-Bernal Juan Luis, Bello-López Miguel Angel, Callejón-Mochón Manuel, Jiménez-Sánchez Juan Carlos, Guiraúm-Pérez A
Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, Seville 41012, Spain.
Talanta. 2005 Feb 15;65(3):686-91. doi: 10.1016/j.talanta.2004.07.030.
Eleven elements (Zn, P, B, Mn, Mg, Cu, Ca, Ba, Sr, Na and K) were determined by inductively plasma coupled spectrometry in 40 honey samples from different places of Spain and four different botanical origins: Eucalyptus (Eucalyptus sp.), Heather (Erica sp.), Orange-blossom (Citrus sinensis) and Rosemary (Rosmarinus officinalis). K, Ca and P show the higher levels with average concentrations ranged between 434.1-1935mgkg(-1) for K; 42.59-341.0mgkg(-1) for Ca and 51.17-154.3mgkg(-1) for P. Levels of Cu (0.531-2.117mgkg(-1)), Ba (0.106-1.264mgkg(-1)) and Sr (0.257-1.462mgkg(-1)) are the lowest in all honey samples. Zn (1.332-7.825mgkg(-1)), Mn (0.133-9.471mgkg(-1)), Mg (13.26-74.38mgkg(-1)) and Na (11.69-218.5mgkg(-1)) concentrations were found strongly dependent on the kind of botanical origin. Results were submitted to pattern recognition procedures, unsupervised methods such as cluster and principal components analysis and supervised learning methods like linear discriminant analysis in order to evaluate the existence of data patterns and the possibility of differentiation of Spanish honeys from different botanical origins according to their mineral content. Cluster analysis shows four clusters corresponding to the four botanical origins of honey and PCA explained 71% of the variance with the first two PC variables. The best-grouped honeys were those from heather; eucalyptus honeys formed a more dispersed group and finally orange-blossom and rosemary honeys formed a less distinguishable group.
采用电感耦合等离子体质谱法测定了来自西班牙不同地区、4种不同植物源（桉属植物、石南属植物、柑橘属植物和迷迭香）的40份蜂蜜样品中的11种元素（锌、磷、硼、锰、镁、铜、钙、钡、锶、钠和钾）。钾、钙和磷含量较高，钾的平均浓度在434.1 - 1935mg/kg之间；钙为42.59 - 341.0mg/kg；磷为51.17 - 154.3mg/kg。所有蜂蜜样品中，铜（0.531 - 2.117mg/kg）、钡（0.106 - 1.264mg/kg）和锶（0.257 - 1.462mg/kg）的含量最低。锌（1.332 - 7.825mg/kg）、锰（0.133 - 9.471mg/kg）、镁（13.26 - 74.38mg/kg）和钠（11.69 - 218.5mg/kg）的浓度强烈依赖于植物源种类。将结果进行模式识别分析，采用无监督方法如聚类分析和主成分分析，以及有监督学习方法如线性判别分析，以评估数据模式的存在情况，以及根据矿物质含量区分不同植物源西班牙蜂蜜的可能性。聚类分析显示有4个聚类，分别对应蜂蜜的4种植物源，主成分分析中前两个主成分变量解释了71%的方差。分组效果最好的是石南属植物蜂蜜；桉属植物蜂蜜形成了一个更为分散的组，最后柑橘属植物和迷迭香蜂蜜形成了一个较难区分的组。
