Baytak Sıtkı, Türker A Rehber
Deparment of Chemistry, Science and Art Faculty, Harran University, TR-63100 Sanliurfa, Turkey.
Talanta. 2005 Feb 28;65(4):938-45. doi: 10.1016/j.talanta.2004.08.021.
A microorganism Agrobacterium tumefacients as an immobilized cell on a solid support was presented as a new biosorbent for the enrichment of Fe(III), Co(II), Mn(II) and Cr(III) prior to flame atomic absorption spectrometric analysis. Amberlite XAD-4 was used as a support material for column preconcentration. Various parameters such as pH, amount of adsorbent, eluent type and volume, flow rate of sample solution, volume of sample solution and matrix interference effect on the retention of the metal ions have been studied. The optimum pH for the sorption of above mentioned metal ions were about 6, 8, 8 and 6, respectively. The loading capacity of adsorbent for Co(II) and Mn(II) were found to be 29 and 22mumolg(-1), respectively. The recoveries of Fe(III), Co(II), Mn(II) and Cr(III), under the optimum conditions were found to be 99 +/- 3, 99 +/- 2, 98 +/- 3 and 98 +/- 3%, respectively, at the 95% confidence level. The limit of detection was 3.6, 3.0, 2.8 and 3.6ngml(-1) for Fe(III), Co(II), Mn(II) and Cr (III), respectively, by applying a preconcentration factor of 25. The proposed enrichment method was applied for metal ion determination from water samples, alloy samples, infant foods and certified samples such as whey powder (IAEA-155) and aluminum alloy (NBS SRM 85b). The analytes were determined with a relative error lower than 10% in all samples.
一种固定在固体载体上的微生物根癌土壤杆菌被作为一种新型生物吸附剂提出，用于在火焰原子吸收光谱分析之前富集铁（III）、钴（II）、锰（II）和铬（III）。采用Amberlite XAD - 4作为柱预富集的载体材料。研究了诸如pH值、吸附剂用量、洗脱剂类型和体积、样品溶液流速、样品溶液体积以及基质干扰对金属离子保留的影响等各种参数。上述金属离子吸附的最佳pH值分别约为6、8、8和6。发现吸附剂对钴（II）和锰（II）的负载量分别为29和22μmol g⁻¹。在最佳条件下，铁（III）、钴（II）、锰（II）和铬（III）的回收率在95%置信水平下分别为99±3%、99±2%、98±3%和98±3%。通过应用25的预富集因子，铁（III）、钴（II）、锰（II）和铬（III）的检测限分别为3.6、3.0、2.8和3.6 ng mL⁻¹。所提出的富集方法应用于水样、合金样品、婴儿食品以及认证样品如乳清粉（IAEA - 155）和铝合金（NBS SRM 85b）中金属离子的测定。所有样品中分析物的测定相对误差均低于10%。
