一种用于毛细管电泳的共线发光二极管诱导荧光检测器。

A collinear light-emitting diode-induced fluorescence detector for capillary electrophoresis.

作者信息

Yang Bingcheng, Tan Feng, Guan Yafeng

机构信息

Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.

出版信息

Talanta. 2005 Mar 15;65(5):1303-6. doi: 10.1016/j.talanta.2004.09.012.

DOI:10.1016/j.talanta.2004.09.012

PMID:18969945

Abstract

A novel fluorescence detector based on collinear scheme using a brightness light-emitting diode emitting at 470nm as excitation source is described. The detector is assembled by all-solid-state optical-electronic components and coupled with capillary electrophoresis using on-column detection mode. Fluorescein isothiocyanate (FITC) and FITC-labeled amino acids and small molecule peptide as test analyte were used to evaluate the detector. The concentration limit of detection for FITC-labeled phenylalanine was 10nM at a signal-to-noise ratio (S/N) of 3. The system exhibited good linear responses in the range of 1x10(-7) to 2x10(-5)M (R(2)=0.999).

摘要

描述了一种基于共线方案的新型荧光检测器，该检测器使用发射波长为470nm的高亮度发光二极管作为激发源。该检测器由全固态光电组件组装而成，并采用柱上检测模式与毛细管电泳联用。使用异硫氰酸荧光素（FITC）以及FITC标记的氨基酸和小分子肽作为测试分析物来评估该检测器。在信噪比（S/N）为3时，FITC标记的苯丙氨酸的检测浓度限为10nM。该系统在1x10(-7)至2x10(-5)M范围内表现出良好的线性响应（R(2)=0.999）。