[一种用于微尺度流动分析系统的新型发光二极管诱导荧光检测器]

[A novel light-emitting diode-induced fluorescence detector for micro-scale flow analysis system].

作者信息

Yang Bing-Cheng, Tan Feng, Guan Ya-Feng

机构信息

Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Dec;25(12):1995-7.

PMID:16544490

Abstract

A compact light-emitting diode-induced fluorescence detector based on collinear scheme is described. The detector was assembled by all-solid-state optical-electronic components and easily coupled with micro-scale flow analysis system by on-column detection mode. Fluorescein isothiocyanate (FITC) and FITC-labeled amino acids were used as test analytes to evaluate the detector performance by capillary electrophoresis and flow injection analysis techniques. The concentration limit of detection was 10(-8) mol x L(-1) for FITC-labeled phenylalanine at a signal-to-noise ratio (S/N) of 3 (on-column, 0.05 mm i.d.). The system exhibited a good linear response in the range of 1 x 10(-7) -2 x 10(-5) mol x L(-1) (r2 = 0.999). The relative standard deviation (RSD) of 6 repetitive injections on peak heights is less than 3%.

摘要

描述了一种基于共线方案的紧凑型发光二极管诱导荧光检测器。该检测器由全固态光电子元件组装而成，并通过柱上检测模式轻松与微型流动分析系统耦合。使用异硫氰酸荧光素（FITC）和FITC标记的氨基酸作为测试分析物，通过毛细管电泳和流动注射分析技术评估检测器性能。在柱内径为0.05 mm、信噪比（S/N）为3的条件下，FITC标记的苯丙氨酸的检测浓度限为10^(-8) mol·L^(-1)。该系统在1×10^(-7) - 2×10^(-5) mol·L^(-1)范围内表现出良好的线性响应（r2 = 0.999）。6次重复进样峰高的相对标准偏差（RSD）小于3%。